Protein Corona of Nanoparticles: Distinct Proteins Regulate the Cellular Uptake,Biomacromolecules

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Protein Corona of Nanoparticles: Distinct Proteins Regulate the Cellular Uptake
Biomacromolecules ( IF 5.5 ) Pub Date : 2015-04-03 00:00:00 , DOI: 10.1021/acs.biomac.5b00108
Sandra Ritz ₁ , Susanne Schöttler ₁ , Niklas Kotman ₁ , Grit Baier ₁ , Anna Musyanovych ₁ , Jörg Kuharev ₂ , Katharina Landfester ₁ , Hansjörg Schild ₂ , Olaf Jahn ₃ , Stefan Tenzer ₂ , Volker Mailänder _{1,

4}

Affiliation

Understanding nanoparticle–protein interactions is a crucial issue in the development of targeted nanomaterial delivery. Besides unraveling the composition of the nanoparticle’s protein coronas, distinct proteins thereof could control nanoparticle uptake into specific cell types. Here we differentially analyzed the protein corona composition on four polymeric differently functionalized nanoparticles by label-free quantitative mass spectrometry. Next, we correlated the relative abundance of identified proteins in the corona with enhanced or decreased cellular uptake of nanoparticles into human cancer and bone marrow stem cells to identify key candidates. Finally, we verified these candidate proteins by artificially decorating nanoparticles with individual proteins showing that nanoparticles precoated with the apolipoproteins ApoA4 or ApoC3 significantly decreased the cellular uptake, whereas precoating with ApoH increased the cellular uptake.

中文翻译：

纳米粒子的蛋白质电晕：不同的蛋白质调节细胞的摄取。

了解纳米粒子与蛋白质之间的相互作用是开发靶向纳米材料的过程中的关键问题。除了揭示纳米粒子蛋白质电晕的组成外，其独特的蛋白质还可以控制纳米粒子对特定细胞类型的摄取。在这里，我们通过无标记定量质谱分析法对四种聚合的功能不同的纳米颗粒上的蛋白质电晕组成进行了差异分析。接下来，我们将日冕中已鉴定蛋白质的相对丰度与增强或降低纳米粒子对人癌和骨髓干细胞的细胞摄取以鉴定关键候选物相关联。最后，

更新日期：2015-04-03

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