Partial-nitritation anammox (PNA) is a novel wastewater treatment procedure for energy-efficient ammonium removal. Here we use genome-resolved metagenomics to build a genome-based ecological model of the microbial community in a full-scale PNA reactor. Sludge from the bioreactor examined here is used to seed reactors in wastewater treatment plants around the world; however, the role of most of its microbial community in ammonium removal remains unknown. Our analysis yielded 23 near-complete draft genomes that together represent the majority of the microbial community. We assign these genomes to distinct anaerobic and aerobic microbial communities. In the aerobic community, nitrifying organisms and heterotrophs predominate. In the anaerobic community, widespread potential for partial denitrification suggests a nitrite loop increases treatment efficiency. Of our genomes, 19 have no previously cultivated or sequenced close relatives and six belong to bacterial phyla without any cultivated members, including the most complete Omnitrophica (formerly OP3) genome to date.

中文翻译：

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全基因组废水处理系统中基于基因组的厌氧菌颗粒微生物生态学。

部分硝化厌氧氨氧化（PNA）是一种用于高效去除氨的新型废水处理程序。在这里，我们使用基因组解析的宏基因组学来建立全尺寸PNA反应器中微生物群落的基于基因组的生态模型。此处检查的生物反应器产生的污泥用于世界各地废水处理厂的反应堆种子。然而，大多数微生物群落在去除铵中的作用仍然未知。我们的分析产生了23个几乎完整的草图基因组，它们共同代表了大多数微生物群落。我们将这些基因组分配给不同的厌氧和有氧微生物群落。在有氧社区中，硝化生物和异养生物占主导地位。在厌氧社区，部分反硝化的广泛潜力表明亚硝酸盐环增加了处理效率。在我们的基因组中，有19个以前没有经过培养或测序的近亲，有6个属于细菌门而没有任何培养成员，包括迄今为止最完整的全营养菌（以前称为OP3）基因组。