The inconsistent vitality and efficiency of plant growth promoting bacteria (PGPB) are technical limitations in the application of PGPB as biofertilizer. To improve these disadvantages, we examined the potential of micro Dielectric Barrier Discharge (DBD) plasma to enhance the vitality and functional activity of a PGPB, Bacillus subtilis CB-R05. Bacterial multiplication and motility were increased after plasma treatment, and the level of a protein involved in cell division was elevated in plasma treated bacteria. Rice seeds inoculated with plasma treated bacteria showed no significant change in germination, but growth and grain yield of rice plants were significantly enhanced. Rice seedlings infected with plasma treated bacteria showed elevated tolerance to fungal infection. SEM analysis demonstrated that plasma treated bacteria colonized more densely in the broader area of rice plant roots than untreated bacteria. The level of IAA (Indole-3-Acetic Acid) and SA (Salicylic Acid) hormone was higher in rice plants infected with plasma treated than with untreated bacteria. Our results suggest that plasma can accelerate bacterial growth and motility, possibly by increasing the related gene expression, and the increased bacterial vitality improves colonization within plant roots and elevates the level of phytohormones, leading to the enhancement of plant growth, yield, and tolerance to disease.

植物生长细菌（PGPB）的生命力和效率不一致是将PGPB用作生物肥料的技术限制。为了改善这些缺点，我们研究了微介电势垒放电（DBD）等离子体增强PGPB，枯草芽孢杆菌的活力和功能活性的潜力。CB-R05。血浆处理后细菌的繁殖和运动性增加，而血浆处理的细菌中参与细胞分裂的蛋白质水平升高。用等离子处理的细菌接种的水稻种子发芽没有明显变化，但是水稻植物的生长和籽粒产量显着提高。用血浆处理过的细菌感染的水稻幼苗对真菌感染的耐受性升高。SEM分析表明，与未处理的细菌相比，经等离子体处理的细菌在水稻植物根部更广泛的区域中更密集地定居。在用血浆处理过的水稻植株中，IAA（吲哚-3-乙酸）和SA（水杨酸）激素的水平高于未经处理的细菌。我们的结果表明，血浆可以促进细菌的生长和运动，