多孔羟基磷灰石支架与生物活性玻璃/聚乳酸-乙醇酸复合纤维复合纤维的<i>协同</i>增效促进成骨活性和生物活性,ACS Omega

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多孔羟基磷灰石支架与生物活性玻璃/聚乳酸-乙醇酸复合纤维复合纤维的协同增效促进成骨活性和生物活性
ACS Omega ( IF 3.7 ) Pub Date : 2019-01-30 00:00:00 , DOI: 10.1021/acsomega.8b02898
Jeong-Hyun Ryu , Jae-Sung Kwon , Kwang-Mahn Kim , Hye Jin Hong , Won-Gun Koh , Jaejun Lee , Hyo-Jung Lee ₁ , Heon-Jin Choi , Seong Yi ₁ , Hyunjung Shin ₂ , Min-Ho Hong ₂

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多孔羟基磷灰石（HAp）支架由于其生物相容性和骨传导特性而通常用于硬组织再生，但在生物活性和骨诱导方面受到限制。本研究调查了涂有聚乳酸共聚物的HAp支架的制备。-乙醇酸/ 45S5生物活性玻璃（PLGA / BG）复合超细纤维，采用海绵仿制法和静电纺丝工艺，可在成骨过程中改善生物活性和骨诱导性。HAp / PLGA / BG（HPB）支架的表征是通过检查形态和离子释放来进行的。HPB支架的生物学评估是通过使用MC3T3-E1前成骨细胞评估细胞毒性，细胞增殖和细胞分化来进行的。结果表明，所有HPB支架均显示出89.2％的高孔隙率，并具有覆盖有含BG离子（Si–Ca–Na–P）的复合材料层的多孔结构。这些支架可以控制Si，Ca，Na和P离子释放长达28天。支架之间的细胞毒性没有显着差异。从第1天到第3天，HPB支架上的细胞增殖增加。此外，LIVE / DEAD分析证实了HPB支架上的细胞活力。如碱性磷酸酶活性和蛋白质印迹分析所示，细胞分化表明与其他支架相比，具有20和30分钟涂层的HPB支架诱导更高水平的成骨相关标志物。此外，免疫细胞化学表明骨桥蛋白表达。茜素红染色表明，在矿化方面，涂有20分钟涂层的HPB支架比HAp支架更有效。总之，用PLGA / BG涂覆20分钟的HAp支架有望成为具有成骨活性的材料，并且可能是组织工程的潜在骨替代物。如碱性磷酸酶活性和蛋白质印迹分析所示，细胞分化表明与其他支架相比，具有20和30分钟涂层的HPB支架诱导更高水平的成骨相关标志物。此外，免疫细胞化学表明骨桥蛋白表达。茜素红染色表明，在矿化方面，涂有20分钟涂层的HPB支架比HAp支架更有效。总之，用PLGA / BG涂覆20分钟的HAp支架有望成为具有成骨活性的材料，并且可能是组织工程的潜在骨替代物。如碱性磷酸酶活性和蛋白质印迹分析所示，细胞分化表明与其他支架相比，具有20和30分钟涂层的HPB支架诱导更高水平的成骨相关标志物。此外，免疫细胞化学表明骨桥蛋白表达。茜素红染色表明，在矿化方面，涂有20分钟涂层的HPB支架比HAp支架更有效。总之，用PLGA / BG涂覆20分钟的HAp支架有望成为具有成骨活性的材料，并且可能是组织工程的潜在骨替代物。免疫细胞化学表明骨桥蛋白表达。茜素红染色表明，在矿化方面，涂有20分钟涂层的HPB支架比HAp支架更有效。总之，涂有PLGA / BG 20分钟的HAp支架有望成为具有成骨活性的材料，并且可能是组织工程的潜在骨替代物。免疫细胞化学表明骨桥蛋白表达。茜素红染色表明，在矿化方面，涂有20分钟涂层的HPB支架比HAp支架更有效。总之，用PLGA / BG涂覆20分钟的HAp支架有望成为具有成骨活性的材料，并且可能是组织工程的潜在骨替代物。

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Synergistic Effect of Porous Hydroxyapatite Scaffolds Combined with Bioactive Glass/Poly(lactic-co-glycolic acid) Composite Fibers Promotes Osteogenic Activity and Bioactivity

Porous hydroxyapatite (HAp) scaffolds are commonly used for hard tissue regeneration because of their biocompatibility and osteoconduction properties, but they are limited in terms of bioactivity and osteoinduction. This study investigated the fabrication of HAp scaffolds coated with poly(lactic-co-glycolic acid)/45S5 bioactive glass (PLGA/BG) composite microfibers using the sponge replica method and electrospinning process for improved bioactivity and osteoinductivity during osteogenesis. Characterization of the HAp/PLGA/BG (HPB) scaffold was carried out by examining morphology and ion release. The biological evaluation of HPB scaffolds was carried out by assessing cytotoxicity, cell proliferation, and cell differentiation using MC3T3-E1 preosteoblasts. The results showed that all HPB scaffolds exhibited a high porosity of 89.2% and had porous structures coated with a layer of composite containing BG ions (Si–Ca–Na–P). These scaffolds enabled controlled release of Si, Ca, Na, and P ions for up to 28 days. There was no significant difference in cytotoxicity between the scaffolds. Cell proliferation on HPB scaffolds was increased from day 1 to 3. In addition, cell viability on the HPB scaffolds was confirmed with LIVE/DEAD assay. Cell differentiation, as shown by alkaline phosphatase activity and western blot analyses, indicated that HPB scaffolds with 20 and 30 min of coating induced higher levels of osteogenesis-related markers compared to other scaffolds. Furthermore, immunocytochemistry indicated osteopontin expression. Alizarin red staining indicated that HPB scaffolds with 20 min of coating were more effective than the HAp scaffold in terms of mineralization. In conclusion, HAp scaffolds coated with PLGA/BG for 20 min are promising materials for osteogenic activity and may be a potential bone substitute for tissue engineering.

更新日期：2019-01-30

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