Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD+/Dehydrogenase‐based Ethanol Biosensor,Electroanalysis

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Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD+/Dehydrogenase‐based Ethanol Biosensor
Electroanalysis ( IF 2.7 ) Pub Date : 2019-01-30 , DOI: 10.1002/elan.201800892
Pablo Gallay ₁ , Marcos Eguílaz ₁ , Gustavo Rivas ₁

Affiliation

This work reports for the first time the development of a reagentless enzymatic amperometric biosensor for ethanol based on the use of a glassy carbon electrode (GCE) modified with multi‐walled carbon nanotubes (MWCNTs) non‐covalently functionalized with polyarginine (Polyarg) as platform for the robust immobilization of alcohol dehydrogenase (ADH) and NAD⁺. The new strategy allows to obtain an integrated GCE/MWCNTs‐Polyarg/NAD⁺‐ADH ethanol biosensor with important advantages compared to the existing ethanol biosensors: avoids the external addition of the cofactor for each measurement, ensures a fast and sensitive quantification of ethanol due to the intimate interaction of the components, and allows the detection at considerably lower potentials due to the catalytic activity of the carbon nanostructures. These unique properties have made possible a very efficient ethanol quantification with a sensitivity of (1487±6) μA M⁻¹, detection limit of 0.65 μM, response time of 8 s, and reproducibility of 5.5 % with a very successful application for the quantification of ethanol in different commercial beverages.

中文翻译：

聚精氨酸非共价功能的多壁碳纳米管：构建基于无试剂的基于NAD + /脱氢酶的乙醇生物传感器的新选择

这项工作首次报告了基于乙醇的无试剂酶安培生物传感器的开发，该传感器使用修饰有多价碳纳米管（MWCNT）的玻碳电极（GCE），该碳纳米管未以多聚精氨酸（Polyarg）共价功能化用于酒精脱氢酶（ADH）和NAD ⁺的牢固固定。新策略允许获得集成的GCE / MWCNTs-Polyarg / NAD ⁺‐ADH乙醇生物传感器与现有的乙醇生物传感器相比具有重要优势：避免每次测量都额外添加辅因子，由于各组分之间的紧密相互作用，可确保对乙醇进行快速灵敏的定量，并允许以低得多的电位进行检测由于碳纳米结构的催化活性。这些独特的特性使高效的乙醇定量成为可能，灵敏度为（1487±6）μAM ^-1，检测限为0.65μM，响应时间为8 s，重现性为5.5％，非常成功地应用于定量不同商业饮料中的乙醇含量

更新日期：2019-01-30

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