2-Hydroxy-3-methylanthraquinone 通过调节 IL-6 诱导的 JAK2/STAT3 通路抑制肺癌细胞。,Phytomedicine

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2-Hydroxy-3-methylanthraquinone 通过调节 IL-6 诱导的 JAK2/STAT3 通路抑制肺癌细胞。
Phytomedicine ( IF 6.7 ) Pub Date : 2019-01-28 , DOI: 10.1016/j.phymed.2019.152848
Chao Sun ₁ , Jing Yang ₂ , Hai-Bo Cheng ₃ , Wei-Xing Shen ₃ , Ze-Qun Jiang ₂ , Ming-Jie Wu ₄ , Li Li ₂ , Wen-Ting Li ₂ , Ting-Ting Chen ₃ , Xi-Wu Rao ₃ , Jin-Rong Zhou ₅ , Mian-Hua Wu ₂

Affiliation

Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Institute of Oncology, the First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Translational Medicine Research Center, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Nutrition/Metabolism Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave, Boston, MA 02215, USA.
Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Institute of Oncology, the First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Translational Medicine Research Center, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
Nutrition/Metabolism Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave, Boston, MA 02215, USA.

背景技术2-羟基-3-甲基蒽醌(HMA)是中药白花蛇舌草中的一种蒽醌单体，据报道可抑制多种癌症的生长，但其对肺癌的作用尚未得到充分研究。假设/目的本研究旨在验证 HMA 部分通过下调白介素 (IL)-6 诱导的 JAK2/STAT3 通路抑制肺癌细胞生长、迁移和侵袭的假设。方法分别采用CCK-8法和Annexin V-FITC/PI流式细胞仪检测肺癌细胞的生长和凋亡。A549细胞的迁移和侵袭分别通过伤口愈合试验和transwell侵袭试验确定。通过细胞因子抗体阵列测定评估 HMA 对 A549 细胞中细胞因子表达的影响。分别通过实时PCR和Western印迹分析定量相关分子标记的基因表达和蛋白质水平。结果 HMA 显着抑制 IL-6 刺激的 A549 细胞的生长和集落形成，增加凋亡细胞的数量，并抑制与 IL-6 诱导的 MMP-1、MMP-2 和 MMP-9 表达下调相关的侵袭基因。IL-6 增加了 A549 细胞中 JAK2 和 STAT3 的酪氨酸磷酸化水平，这被 HMA 处理逆转。此外，HMA降低了A549细胞上清液中一系列炎症相关细胞因子的表达，包括IL-6、G-CSF、IL-6R、IL-8、MCP-1、RANTES、TNF-α。结论这些结果表明，HMA可能部分通过下调IL-6诱导的JAK2/STAT3通路来抑制肺癌细胞的生长和侵袭。

"点击查看英文标题和摘要"

2-Hydroxy-3-methylanthraquinone inhibits lung carcinoma cells through modulation of IL-6-induced JAK2/STAT3 pathway.

BACKGROUND 2-hydroxy-3-methylanthraquinone (HMA), an anthraquinone monomer in traditional Chinese medicine Hedyotis diffusa, has been reported to inhibit the growth of several types of cancer, but its effect on lung cancer has not been adequately investigated. HYPOTHESIS/PURPOSE This study aimed to test the hypothesis that HMA inhibit the growth, migration, and invasion of lung cancer cells in part via downregulation of interleukin (IL)-6-induced JAK2/STAT3 pathway. METHODS Growth and apoptosis of lung cancer cells were quantitated by CCK-8 assay and Annexin V-FITC/PI flow cytometric analysis, respectively. Migration and invasion of A549 cells were determined by wound-healing assay and transwell invasion assay, respectively. The effect of HMA on cytokines expression in A549 cells was evaluated by the cytokine antibody array assay. Gene expression and protein levels of related molecular markers were quantitated by real time-PCR and Western blot analysis, respectively. RESULTS HMA significantly inhibited IL-6-stimulated growth and colony formation of A549 cells, increased the number of apoptotic cells, and inhibited invasion associated with downregulation of expression of IL-6-induced MMP-1, MMP-2, and MMP-9 genes. IL-6 increased the levels of tyrosine phosphorylation of JAK2 and STAT3 in A549 cells, which was reversed by HMA treatment. In addition, HMA reduced the expression of a series of inflammation-related cytokines in A549 cells supernatant, including IL-6, G-CSF, IL-6R, IL-8, MCP-1, RANTES, TNF-α. CONCLUSION These results suggest that HMA may inhibit the growth and invasion of lung cancer cells in part via downregulation of IL-6-induced JAK2/STAT3 pathway.

更新日期：2019-01-28

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