The antifungal mechanism of essential oils against fungi remains in the shallow study. In this paper, antifungal mechanism of trans-cinnamaldehyde against Penicillium italicum was explored. Trans-cinnamaldehyde exhibited strong mycelial growth inhibition against Penicillium italicum, with minimum inhibitory concentration of 0.313 μg/mL. Conventional analytical tests showed that trans-cinnamaldehyde changed the cell membrane permeability, which led to the leakage of some materials. Meanwhile, the membrane integrity and cell wall integrity also changed. Surface-enhanced Raman spectroscopy, an ultrasensitive and fingerprint method, was served as a bran-new method to study the antifungal mechanism. Characteristic peaks of supernatant obviously changed at 734, 1244, 1330, 1338 and 1466 cm⁻¹. The Raman intensity represented a strong correlation with results from conventional methods, which made SERS an alternative to study antifungal process. All evidences implied that trans-cinnamaldehyde exerts its antifungal capacity against Penicillium italicum via membrane damage mechanism.

精油对真菌的抗真菌机制仍在浅层研究中。本文研究了反式肉桂醛对意大利青霉的抗真菌作用机理。反式肉桂醛对意大利青霉菌具有强烈的菌丝生长抑制作用，最低抑制浓度为0.313μg/ mL。常规分析测试表明，反式肉桂醛会改变细胞膜的通透性，从而导致某些物质的泄漏。同时，膜的完整性和细胞壁的完整性也发生了变化。表面增强拉曼光谱，一种超灵敏的指纹方法，被用作研究抗真菌机理的全新方法。上清液的特征峰在734、1244、1330、1338和1466 cm处发生明显变化^-1。拉曼强度与常规方法的结果具有很强的相关性，这使SERS成为研究抗真菌过程的替代方法。所有证据表明反式肉桂醛通过膜破坏机制发挥了对Italicum青霉的抗真菌能力。