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Visualization of Sulfane Sulfur in Plants with a Near-Infrared Fluorescent Probe.
ACS Sensors ( IF 8.2 ) Pub Date : 2019-02-05 , DOI: 10.1021/acssensors.8b01423 Gangwei Jiang 1 , Man Li 1 , Yueyan Wen 1 , Weili Zeng 1 , Qing Zhao 2 , Chunli Chen 2 , Hong Yuan 1 , Chunrong Liu 1 , Changlin Liu 1
ACS Sensors ( IF 8.2 ) Pub Date : 2019-02-05 , DOI: 10.1021/acssensors.8b01423 Gangwei Jiang 1 , Man Li 1 , Yueyan Wen 1 , Weili Zeng 1 , Qing Zhao 2 , Chunli Chen 2 , Hong Yuan 1 , Chunrong Liu 1 , Changlin Liu 1
Affiliation
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Sulfane sulfur species are an important type of reactive sulfur species. These compounds have unique reactivity to attach reversibly to other sulfur atoms and exhibit regulatory effects in diverse biological systems. Recent studies have suggested that sulfane sulfurs are involved in signal transduction processes of hydrogen sulfide (H2S). The development of probes for selective, rapid, and sensitive detection of sulfane sulfur is of great significance for studying their physiological and pathological roles in biological systems, especially in plant systems for which physiological research has lagged behind. However, so far there is still a lack of sufficient chemical tools for directly tracking and measuring sulfane sulfur in biological systems, and in particular, the detection of sulfane sulfur in living plant tissues is still challenging. Herein, we report a near-infrared fluorescent probe, SSNIP, for the selective imaging of sulfane sulfur. SSNIP is capable of detecting sulfane sulfur at physiological concentrations in both aqueous buffer and living human cells. Then, with SSNIP, we demonstrate the fluorescent monitoring of endogenous sulfane sulfur in plant tissues such as Arabidopsis thaliana roots for the first time. Furthermore, the application of SSNIP in evaluating the level of sulfane sulfur in Arabidopsis thaliana roots at different growth stages is performed. The results show that the level of sulfane sulfur in Arabidopsis thaliana roots correlates well with their growth stages, which suggests that sulfane sulfurs might act as actual signaling molecules to promote plant growth and root elongation. In addition, it reveals potential applications for the biological and pathological studies of sulfane sulfur, especially in plant physiology.
中文翻译：
用近红外荧光探针可视化植物中的硫磺硫。
硫磺硫是反应性硫的重要类型。这些化合物具有独特的反应性，可逆地与其他硫原子相连，并在多种生物系统中发挥调节作用。最近的研究表明，硫磺硫与硫化氢（H2S）的信号转导过程有关。开发用于选择性，快速和灵敏检测亚砜硫的探针，对于研究其在生物系统中的生理和病理作用，特别是在生理学研究落后的植物系统中，具有重要意义。然而，迄今为止，仍然缺乏足够的化学工具来直接追踪和测量生物系统中的硫磺硫，特别是在活体植物组织中对硫磺硫的检测仍然具有挑战性。在此处，我们报告了一种用于硫磺选择性成像的近红外荧光探针SSNIP。SSNIP能够在水性缓冲液和活人细胞中检测出生理浓度的亚砜硫。然后，利用SSNIP，我们首次展示了植物组织如拟南芥根中内源性硫烷硫的荧光监测。此外，还进行了SSNIP在评估不同生长阶段拟南芥根中亚砜硫含量方面的应用。结果表明，拟南芥根中的硫磺水平与其生长阶段密切相关，这表明硫磺可能是促进植物生长和根系伸长的实际信号分子。此外，
更新日期：2019-01-25
中文翻译：
用近红外荧光探针可视化植物中的硫磺硫。
硫磺硫是反应性硫的重要类型。这些化合物具有独特的反应性，可逆地与其他硫原子相连，并在多种生物系统中发挥调节作用。最近的研究表明，硫磺硫与硫化氢（H2S）的信号转导过程有关。开发用于选择性，快速和灵敏检测亚砜硫的探针，对于研究其在生物系统中的生理和病理作用，特别是在生理学研究落后的植物系统中，具有重要意义。然而，迄今为止，仍然缺乏足够的化学工具来直接追踪和测量生物系统中的硫磺硫，特别是在活体植物组织中对硫磺硫的检测仍然具有挑战性。在此处，我们报告了一种用于硫磺选择性成像的近红外荧光探针SSNIP。SSNIP能够在水性缓冲液和活人细胞中检测出生理浓度的亚砜硫。然后，利用SSNIP，我们首次展示了植物组织如拟南芥根中内源性硫烷硫的荧光监测。此外，还进行了SSNIP在评估不同生长阶段拟南芥根中亚砜硫含量方面的应用。结果表明，拟南芥根中的硫磺水平与其生长阶段密切相关，这表明硫磺可能是促进植物生长和根系伸长的实际信号分子。此外，
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