Electrochemiluminescence (ECL) has emerged as one of the most important methods for in vitro diagnosis and detection, but it is still limited in sensitivity for ultrasensitive biodetections. Fast and ultrasensitive detection of biomolecules is critical, especially for the clinical detection of cardiac troponin I (cTnI) for cardiac infarction diagnosis. In this study, an effective tactic was developed to enhance ECL efficiency of the luminol system, by combined use of Co²⁺-based metal organic frameworks (MOF), zeolitic imidazolate frameworks (ZIF-67), and luminol-capped Ag nanoparticles (luminol-AgNPs). The integration leads to a pronounced ∼115-fold enhancement in luminol ECL. On the basis of this fascinating sensing platform, a robust label-free ECL immunosensor was constructed for ultrasensitive detection of cTnI, the main marker of myocardial infarction, with good stability and a detection limit as low as 0.58 fg mL^–1 (S/N = 3).

中文翻译：

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钴基金属有机骨架和银纳米粒子的结合使用增强鲁米诺电化学发光及其在心肌肌钙蛋白I的超灵敏检测中的应用。

电化学发光（ECL）已经成为体外诊断和检测的最重要方法之一，但对于超灵敏生物检测的灵敏度仍然受到限制。快速，超灵敏地检测生物分子至关重要，特别是对于临床检测心肌肌钙蛋白I（cTnI）来诊断心肌梗塞至关重要。在这项研究中，通过结合使用Co ²⁺，开发了一种有效的策略来提高Luminol系统的ECL效率。的金属有机骨架（MOF），沸石咪唑酸酯骨架（ZIF-67）和以鲁米诺封端的Ag纳米粒子（luminol-AgNPs）。整合导致鲁米诺ECL明显提高〜115倍。在这个引人入胜的传感平台的基础上，构建了功能强大的无标记ECL免疫传感器，用于cTnI（心肌梗死的主要标志物）的超灵敏检测，具有良好的稳定性，检出限低至0.58 fg mL ^–1（S / N = 3）。