The compound, 1-((4-fluorophenyl)thio)isoquinoline (FPTQ), is a synthetic isoquinoline derivative. To test the anti-inflammatory effect of FPTQ, we used neutrophil-specific transgenic zebrafish Tg(mpx::EGFP)ⁱ¹¹⁴ line and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We also used two different methods, involving tail transection and LPS stimulation in the zebrafish model. Neutrophils translocation in the zebrafish tail-transected model was inhibited by FPTQ. Neutrophil aggregation was also inhibited by FPTQ in the LPS-stimulated zebrafish model. Decreased mRNA expression of the pro-inflammatory cytokine genes, interleukin-1β (il-1β) and interleukin-6 (il-6), was found in zebrafish larvae injected with FPTQ. Additionally, production of nitric oxide was inhibited by FPTQ in RAW264.7 macrophage cells treated with LPS. Moreover, the mRNA expression of Il-1β and Il-6 suppressed by FPTQ treatment in RAW264.7 macrophage cells, and an enzyme immunoassay showed that FPTQ suppressed the secretion of IL-1β and IL-6 in RAW264.7 cells. These results demonstrate that FPTQ reduced inflammatory responses and, therefore, suggest that it may be effective as an anti-inflammatory agent.

化合物1-（（4-氟苯基）硫基）异喹啉（FPTQ）是合成的异喹啉衍生物。为了测试FPTQ的抗炎作用，我们使用了嗜中性粒细胞特异性转基因斑马鱼Tg（mpx :: EGFP）ⁱ¹¹⁴系和脂多糖（LPS）刺激的RAW264.7细胞。我们还使用了两种不同的方法，包括在斑马鱼模型中进行尾部横断和LPS刺激。FPTQ抑制斑马鱼尾部切除模型中的中性粒细胞移位。在LPS刺激的斑马鱼模型中，FPTQ也抑制了中性粒细胞的聚集。促炎细胞因子基因白细胞介素-1β（il-1β）和白细胞介素6（il-6）的mRNA表达降低，在注射FPTQ的斑马鱼幼虫中被发现。此外，在用LPS处理的RAW264.7巨噬细胞中，FPTQ抑制一氧化氮的产生。此外，通过FPTQ处理在RAW264.7巨噬细胞中抑制了Il-1β和Il-6的mRNA表达，并且酶免疫测定表明FPTQ抑制了RAW264.7细胞中IL-1β和IL-6的分泌。这些结果证明FPTQ减少了炎症反应，因此表明它可以作为抗炎剂有效。