Enzyme-mediated intercellular proximity labeling for detecting cell-cell interactions,Journal of the American Chemical Society

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Enzyme-mediated intercellular proximity labeling for detecting cell-cell interactions
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2019-01-24 , DOI: 10.1021/jacs.8b10286
Yun Ge ₁ , Long Chen ₁ , Shibo Liu ₁ , Jingyi Zhao ₁ , Heng Zhang ₁ , Peng R Chen _{1,

2}

Affiliation

Cell-cell interactions and communications play fundamental roles in life processes but remain largely uncharacterized. We developed an enzyme-mediated proximity cell labeling (EXCELL) strategy as a general method to detect and record cell-cell interactions under living conditions. EXCELL relies on an evolved Staphylococcus aureus transpeptidase sortase A variant (mgSrtA) capable of promiscuous labeling of various cell surface proteins containing a monoglycine residue at the N-terminus. Displaying mgSrtA on the surface of a cell of interest allows the labeling and detection of interacting cells in a proximity-dependent fashion.

中文翻译：

用于检测细胞间相互作用的酶介导的细胞间邻近标记

细胞间相互作用和通信在生命过程中发挥着重要作用，但在很大程度上仍未得到表征。我们开发了一种酶介导的邻近细胞标记 (EXCELL) 策略，作为在生活条件下检测和记录细胞间相互作用的通用方法。EXCELL 依赖于进化的金黄色葡萄球菌转肽酶分选酶 A 变体 (mgSrtA)，能够混杂标记在 N 端含有单甘氨酸残基的各种细胞表面蛋白。在感兴趣的细胞表面上显示 mgSrtA 允许以接近依赖的方式标记和检测相互作用的细胞。

更新日期：2019-01-24

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