The synthesis and structural characterization of novel mixed ligand complexes derived from 9-anthraldehyde, 2-amino-3-hydroxy pyridine, 1,10-phenanthroline and metal chlorides are reported. The synthesized mixed ligand complexes are characterized by multispectral techniques such as UV‐Visible, FT-IR, EPR, NMR, ESI-mass analysis and other physicochemical analysis like elemental analysis, molar conductivity, magnetic susceptibility measurements. Moreover, the mixed ligand complexes have strived for their biological future. The biological studies involved are DNA interaction (binding and damage), antimicrobial, antiproliferative studies and free radical scavenging ability studies. The DNA interaction studies are accomplished by UV‐Visible absorption titration, viscosity measurement, which exposed that the synthesized compounds could interplay with CT-DNA through non-covalent interaction of phenanthroline co-ligand by the way of intercalative binding mode. Additionally, the antimicrobial assay specified that the mixed ligand complexes are excellent antimicrobial agents against various pathogens. The gel electrophoresis scrutiny clearly evinced that the prominent DNA cleavage activity of the mixed ligand complexes to divide the supercoiled pUC 19 DNA. The in-vitro antiproliferative activities of the mixed ligand complexes are explored on MCF-7, Hep G2, HBL-100 cell lines using an MTT assay. The morphological changes of the nucleus are explored using Hoechst 33258 staining apoptosis assay. The antioxidant possessions manifest that the mixed ligand complexes have desirable proficiency to scavenge the hydroxyl radical than the ligand. The prediction of in-silico ADMET property revealed that the Schiff base ligand possesses appreciable drug-likeness characters according to Lipinski's regulations. The prediction of PASS biological activity explains that the synthesized ligand holding the excellent biological potential against various diseases. Eventually, the molecular docking studies are achieved to comprehend the nature of binding of the synthesized ligand and mixed ligand complexes with COX-2 protein and DNA.

报道了衍生自9-乙醛，2-氨基-3-羟基吡啶，1,10-菲咯啉和金属氯化物的新型混合配体配合物的合成和结构表征。合成的混合配体配合物通过多光谱技术进行表征，例如紫外可见光谱，傅立叶变换红外光谱，EPR，NMR，ESI质量分析和其他理化分析，例如元素分析，摩尔电导率，磁化率测量。而且，混合的配体配合物为它们的生物学未来而努力。涉及的生物学研究包括DNA相互作用（结合和破坏），抗微生物，抗增殖研究和自由基清除能力研究。DNA相互作用研究通过紫外可见吸收滴定，粘度测量，揭示了合成的化合物可以通过插入结合模式通过菲咯啉共配体的非共价相互作用与CT-DNA相互作用。另外，抗微生物试验表明，混合的配体复合物是针对各种病原体的优良抗微生物剂。凝胶电泳检查清楚地表明，混合配体复合物对超螺旋pUC 19 DNA的切割具有显着的DNA切割活性。使用MTT分析在MCF-7，Hep G2，HBL-100细胞系上探索了混合配体复合物的体外抗增殖活性。使用Hoechst 33258染色细胞凋亡测定法探索细胞核的形态变化。抗氧化剂拥有物表明，混合的配体配合物比配体具有所需的清除羟基自由基的能力。的预测硅的ADMET特性表明，根据Lipinski的规定，席夫碱配体具有明显的药物样特征。对PASS生物活性的预测解释了合成的配体具有针对各种疾病的优异的生物潜力。最终，完成了分子对接研究，以了解合成的配体和混合的配体复合物与COX-2蛋白和DNA结合的性质。