Improving orthotopic mouse models of patient-derived breast cancer brain metastases by a modified intracarotid injection method.,Scientific Reports

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Improving orthotopic mouse models of patient-derived breast cancer brain metastases by a modified intracarotid injection method.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-01-24 , DOI: 10.1038/s41598-018-36874-3
Zongming Liu _{1,

2,

3,

4} , Yanzhi Wang _{1,

2} , Sheheryar Kabraji _{1,

5} , Shaozhen Xie _{1,

2} , Peichen Pan _{1,

2} , Zhenning Liu ₃ , Jing Ni _{1,

2} , Jean J Zhao _{1,

2}

Affiliation

Breast cancer brain metastasis (BCBM) remains a major clinical problem. Approximately 10–16% of patients with breast cancer develop brain metastases (BCBM). However, no systemic therapy has gained regulatory approval for the specific treatment of BCBM and this remains an area of persistent, unmet medical need. Rapid, predictive and clinically-relevant animal models are critical to study the biology of brain metastases and to identify effective therapeutic approaches for patients with BCBM. Here, we describe a method for efficient establishment of orthotopic mouse models of patient-derived brain metastases via an improved intracarotid injection protocol that permits tumor cell growth in the unique brain microenvironment without compromising the blood-brain barrier (BBB). We demonstrate that our newly improved models of patient-derived brain metastases recapitulate the histologic, molecular, and genetic characteristics of their matched patient tumor specimens and thus represent a potentially powerful tool for pre-clinical and translational research.

中文翻译：

通过改良的颈动脉内注射法改善患者源性乳腺癌脑转移的原位小鼠模型。

乳腺癌脑转移（BCBM）仍然是主要的临床问题。大约10–16％的乳腺癌患者会发生脑转移（BCBM）。但是，尚无针对BCBM的特殊治疗的全身疗法获得监管部门的批准，这仍然是一个持续且尚未满足的医学需求领域。快速，可预测且具有临床相关性的动物模型对于研究脑转移生物学并确定BCBM患者的有效治疗方法至关重要。在这里，我们描述了一种通过改进的颈动脉内注射方案有效建立患者源性脑转移的原位小鼠模型的方法，该方案允许肿瘤细胞在独特的脑微环境中生长而不会损害血脑屏障（BBB）。

更新日期：2019-01-24

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