卵转铁蛋白改善环磷酰胺诱导的免疫调节功能和肠道菌群的功能障碍,Food & Function

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卵转铁蛋白改善环磷酰胺诱导的免疫调节功能和肠道菌群的功能障碍
Food & Function ( IF 5.1 ) Pub Date : 2019-01-23 00:00:00 , DOI: 10.1039/c8fo02312c
Gaoxiang Zhu _{1,

2,

3,

4,

5} , Yan Jiang _{1,

2,

3,

4,

5} , Yao Yao _{1,

2,

3,

4,

5} , Na Wu _{1,

2,

3,

4,

5} , Jing Luo _{3,

6,

7,

8} , Mingwen Hu _{1,

2,

3,

4,

5} , Yonggang Tu _{1,

2,

3,

4,

5} , Mingsheng Xu _{1,

2,

3,

4,

5}

Affiliation

在这项研究中，研究了卵磷脂转铁蛋白（OVT）对注射环磷酰胺（CP）的小鼠模型中免疫调节功能和肠道微生物功能障碍的调节作用。OVT的免疫调节作用通过酶联免疫吸附测定（ELISA）确定。通过对16S rDNA基因的V3-V4区进行高通量测序来确定肠道微生物的组成。在不同的分类学水平上分析了优势菌群相对丰度的变化。结果表明，OVT减轻了CP引起的免疫功能障碍。OVT可改善脾脏和胸腺指数，并增强肿瘤坏死因子α（TNF-α），白介素10（IL-10）和免疫球蛋白A（IgA）的分泌。此外，OVT增加了Shannon和Simpson的指数，提示增加了肠道菌群的多样性和丰富性。相对丰度Lachnospiraceae_NK4A136_group也增加了。但是，幽门螺杆菌和脱硫弧菌的相对丰度明显降低。这些结果表明，食物衍生的功能成分OVT对机体的免疫调节具有影响，并改善了CP诱导的肠道菌群失调，这可通过靶向肠道微生物组来提供禽蛋的潜在治疗用途。

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Ovotransferrin ameliorates the dysbiosis of immunomodulatory function and intestinal microbiota induced by cyclophosphamide

In this study, the regulative effects of ovotransferrin (OVT) on immunomodulatory function and intestinal microbial dysbiosis in a mouse model injected with cyclophosphamide (CP) were investigated. The immunomodulatory effect of OVT was determined by enzyme-linked immune sorbent assay (ELISA). Gut microbial composition was determined by high-throughput sequencing of the V3–V4 region of the 16S rDNA gene. The changes in the relative abundance of the dominant microbiota were analyzed at different taxonomic levels. The results showed that OVT alleviated the immune dysfunction caused by CP. OVT improved the spleen and thymus indices and enhanced the secretion of tumor necrosis factor alpha (TNF-α), interleukin-10 (IL-10), and immunoglobulin A (IgA). In addition, OVT increased the indexes of Shannon and Simpson, suggesting the enhancement of the diversity and richness of intestinal microflora. The relative abundance of Lachnospiraceae_NK4A136_group was also increased. However, the relative abundance of Helicobacter and Desulfovibrio was significantly decreased. These results indicated that OVT, a food-derived functional component, has effects on immune regulation in the organism and ameliorates the gut microbiota disorders induced by CP, which provides a potential therapeutic utilization of avian eggs by targeting the gut microbiome.

更新日期：2019-01-23

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