Sensitive colorimetric sensor for point-of-care detection of acetylcholinesterase using cobalt oxyhydroxide nanoflakes.,Journal of Materials Chemistry B

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Sensitive colorimetric sensor for point-of-care detection of acetylcholinesterase using cobalt oxyhydroxide nanoflakes.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2019-01-28 , DOI: 10.1039/c8tb02987c
Rui Jin ₁ , Zihao Xing , Deshuai Kong , Xu Yan , Fangmeng Liu , Yuan Gao , Peng Sun , Xishuang Liang , Geyu Lu

Affiliation

Point-of-care monitoring of acetylcholinesterase (AChE) is of significant importance for pesticide poisoning and disease diagnosis because it plays a pivotal role in biological nerve conduction systems. Herein, we designed a colorimetric strategy for the facile and accurate detection of AChE based on tandem catalysis with a multi-enzyme system, which is constituted by cobalt oxyhydroxide nanoflakes (CoOOH NFs) and choline oxidase (CHO). In this sensor, AChE catalytically hydrolyzed acetylcholine (ACh) to produce choline, which was further efficiently oxidized by CHO to yield H2O2. CoOOH NFs, as a nanozyme, efficiently catalyzed 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxTMB with the help of H2O2, accompanied by an enhancement of absorbance intensity. The resulting intensity could be employed as the signal output of the CHO/CoOOH/ACh system in monitoring AChE. Under optimal conditions, the developed sensor possessed a sensitive response to AChE with a detection limit of 33 μU mL-1. Interestingly, the proposed platform was applied to fabricate a paper-based sensor for rapidly recognizing AChE by direct observation with the naked eyes. Combined with a smartphone and ImageJ software, we further developed an image-processing algorithm for the quantitative detection of AChE with highly promising results, which validated the outstanding potential of on-site application in clinical diagnostics and pesticide poisoning.

中文翻译：

灵敏的比色传感器，用于使用羟基氧化钴纳米薄片即时检测乙酰胆碱酯酶。

即时点监测乙酰胆碱酯酶（AChE）对于农药中毒和疾病诊断非常重要，因为它在生物神经传导系统中起着关键作用。在本文中，我们设计了一种比色策略，该方法基于多酶系统的串联催化，可轻松，准确地检测AChE，该酶由羟基氧化钴纳米薄片（CoOOH NFs）和胆碱氧化酶（CHO）组成。在该传感器中，AChE催化水解乙酰胆碱（ACh）以产生胆碱，胆碱被CHO进一步有效氧化以生成H2O2。CoOOH NFs作为一种纳米酶，借助H2O2可以有效地将3,3'，5,5'-四甲基联苯胺（TMB）催化成蓝色oxTMB，同时增强了吸收强度。所得强度可用作监测AChE的CHO / CoOOH / ACh系统的信号输出。在最佳条件下，开发的传感器对AChE敏感，检测限为33μUmL-1。有趣的是，所提出的平台被用于制造纸质传感器，以通过肉眼直接观察快速识别AChE。结合智能手机和ImageJ软件，我们进一步开发了用于AChE定量检测的图像处理算法，并获得了非常有希望的结果，验证了现场应用在临床诊断和农药中毒方面的巨大潜力。拟议的平台被用于制造纸质传感器，通过肉眼直接观察快速识别AChE。结合智能手机和ImageJ软件，我们进一步开发了用于AChE定量检测的图像处理算法，并获得了非常有希望的结果，验证了现场应用在临床诊断和农药中毒方面的巨大潜力。拟议的平台被用于制造纸质传感器，通过肉眼直接观察快速识别AChE。结合智能手机和ImageJ软件，我们进一步开发了用于AChE定量检测的图像处理算法，并获得了非常有希望的结果，验证了现场应用在临床诊断和农药中毒方面的巨大潜力。

更新日期：2019-01-14

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