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Dietary Fructooligosaccharide and Glucomannan Alter Gut Microbiota and Improve Bone Metabolism in Senescence-Accelerated Mouse
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-01-11 00:00:00 , DOI: 10.1021/acs.jafc.8b05164 Kenichi Tanabe 1 , Sadako Nakamura 2 , Mie Moriyama-Hashiguchi , Miho Kitajima , Hiroyuki Ejima , Chiaki Imori , Tsuneyuki Oku 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-01-11 00:00:00 , DOI: 10.1021/acs.jafc.8b05164 Kenichi Tanabe 1 , Sadako Nakamura 2 , Mie Moriyama-Hashiguchi , Miho Kitajima , Hiroyuki Ejima , Chiaki Imori , Tsuneyuki Oku 2
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Gut microbiota improved using prebiotics may delay the onset of senescence-related health problems. We hypothesized that prolonged intake of prebiotics delays senile osteoporosis. Forty-five male senescence-accelerated mouse prone 6 (SAMP6) aged four weeks were raised on 5% fructooligosaccharide (FOS), 5% glucomannan (GM), or a control diet for 31 weeks. Gut microbiota were identified using culture-dependent analytical methods. Mineral content in femoral bone was analyzed using atomic absorption spectrophotometry. Bone metabolism and inflammatory markers were measured using enzyme-linked immunosorbent assay. The numbers of Lactobacillus and Bacteroides in cecal contents were significantly higher in the FOS than in the control group (p < 0.05); the number of Clostridium was significantly higher in the GM than in the control group (p < 0.05). Calcium content was significantly higher in the femoral bones of the FOS group (30.5 ± 0.8 mg) than in the control group (27.5 ± 1.5 mg) (p < 0.05). There was no difference between the GM (29.1 ± 2.0 mg) and control groups. During senescence, urinary deoxypyridinoline and serum high-sensitivity C-reactive protein levels significantly decreased in the FOS (1.2 ± 0.2 nmol/3 d and 80 ± 6.1 ng/100 mL) and GM groups (1.2 ± 0.2 nmol/3 d and 80 ± 6.1 ng/100 mL) compared with the control group (1.8 ± 0.5 nmol/3 d and 93 ± 7.4 ng/100 mL) (p < 0.05). Thus, dietary FOS and GM modified gut microbiota and reduced bone resorption by reducing systemic inflammation in SAMP6.
中文翻译：
饮食中低聚果糖和葡甘露聚糖可改变肠道菌群并改善衰老加速小鼠的骨代谢
使用益生元改善肠道菌群可能会延迟与衰老相关的健康问题的发作。我们假设长时间摄入益生元会延缓老年性骨质疏松症。四周龄的四十五只雄性衰老加速小鼠倾向于6（SAMP6）在5％低聚果糖（FOS），5％葡甘露聚糖（GM）或对照饮食下饲养31周。使用依赖于培养物的分析方法鉴定肠道菌群。使用原子吸收分光光度法分析股骨中的矿物质含量。使用酶联免疫吸附测定法测量骨代谢和炎性标志物。FOS组盲肠中乳杆菌和拟杆菌的数量明显高于对照组（p <0.05）。的数量GM中的梭菌明显高于对照组（p <0.05）。FOS组股骨中的钙含量（30.5±0.8 mg）明显高于对照组（27.5±1.5 mg）（p <0.05）。GM（29.1±2.0 mg）和对照组之间没有差异。在衰老期间，FOS（1.2±0.2 nmol / 3 d和80±6.1 ng / 100 mL）和GM组（1.2±0.2 nmol / 3 d和80 d）尿中的脱氧吡啶并啉和血清高敏C反应蛋白水平显着降低与对照组（1.8±0.5 nmol / 3 d和93±7.4 ng / 100 mL）相比，差异为±6.1 ng / 100 mL）（p <0.05）。因此，饮食中的FOS和GM可以通过减少SAMP6中的全身性炎症来改善肠道菌群并减少骨吸收。
更新日期：2019-01-11
中文翻译：
饮食中低聚果糖和葡甘露聚糖可改变肠道菌群并改善衰老加速小鼠的骨代谢
使用益生元改善肠道菌群可能会延迟与衰老相关的健康问题的发作。我们假设长时间摄入益生元会延缓老年性骨质疏松症。四周龄的四十五只雄性衰老加速小鼠倾向于6（SAMP6）在5％低聚果糖（FOS），5％葡甘露聚糖（GM）或对照饮食下饲养31周。使用依赖于培养物的分析方法鉴定肠道菌群。使用原子吸收分光光度法分析股骨中的矿物质含量。使用酶联免疫吸附测定法测量骨代谢和炎性标志物。FOS组盲肠中乳杆菌和拟杆菌的数量明显高于对照组（p <0.05）。的数量GM中的梭菌明显高于对照组（p <0.05）。FOS组股骨中的钙含量（30.5±0.8 mg）明显高于对照组（27.5±1.5 mg）（p <0.05）。GM（29.1±2.0 mg）和对照组之间没有差异。在衰老期间，FOS（1.2±0.2 nmol / 3 d和80±6.1 ng / 100 mL）和GM组（1.2±0.2 nmol / 3 d和80 d）尿中的脱氧吡啶并啉和血清高敏C反应蛋白水平显着降低与对照组（1.8±0.5 nmol / 3 d和93±7.4 ng / 100 mL）相比，差异为±6.1 ng / 100 mL）（p <0.05）。因此，饮食中的FOS和GM可以通过减少SAMP6中的全身性炎症来改善肠道菌群并减少骨吸收。
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