Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Autonomous Sequences in Myoglobin Emerging from X-ray Structure of Holomyoglobin.
ACS Omega ( IF 3.7 ) Pub Date : 2019-01-11 , DOI: 10.1021/acsomega.8b03218 Mitsuaki Narita 1 , Masakuni Narita 2 , Yasuko Itsuno 3 , Shinichi Itsuno 3
ACS Omega ( IF 3.7 ) Pub Date : 2019-01-11 , DOI: 10.1021/acsomega.8b03218 Mitsuaki Narita 1 , Masakuni Narita 2 , Yasuko Itsuno 3 , Shinichi Itsuno 3
Affiliation
|
The proposed continuous folding structure units are fundamental to analyze protein structure. Here, we could elucidate for the first time two types of hydrophobic core networks in apomyoglobin using continuous folding structure units. In myoglobin, two autonomous sequences emerged clearly. We could thus characterize the autonomous sequences using well-defined hydrophobic core networks within respective semifolds. A hydrophobic core is defined as a pair of topology-local hydrophobic amino acids in different folding structures. Hydrophobic core formation is indispensable to stabilize the different folding structures via an efficient hydrophobic interaction. Autonomous sequences in myoglobin encode tertiary structure information for semifolds. These sequences fold autonomously into small sets of continuous folding structure units to grow separate semifolds on each separate framework. The autonomous sequence can be defined as the local sequence assigned to the small set of continuous folding structure units. They create the discrete hydrophobic region in a semifold by assembly of their hydrophobic regions. Semifolds were characterized by discrete hydrophobic regions stabilized by respective type I hydrophobic core networks, which were present within each semifold. The discrete hydrophobic region of a semifold propagated itself with that of a different semifold by hydrophobic interactions in type II hydrophobic core network, which was present between different semifolds, as observed by the X-ray structures of semifolds. The most significant feature of semifolds in apomyoglobin was that they could be verified by the X-ray structure of holomyoglobin regardless of the instability of folds characteristic to autonomous sequence fragments. This work presents the first description of autonomous sequences.
中文翻译：
肌肉红蛋白的X射线结构中出现的肌红蛋白自主序列。
所提出的连续折叠结构单元是分析蛋白质结构的基础。在这里，我们首次使用连续折叠结构单元阐明了肌红蛋白中的两种疏水性核心网络。在肌红蛋白中，清楚地出现了两个自主序列。因此，我们可以在各个半折叠内使用定义明确的疏水性核心网络来表征自主序列。疏水核定义为在不同折叠结构中的一对拓扑局部疏水氨基酸。疏水核的形成对于通过有效的疏水相互作用稳定不同折叠结构必不可少。肌红蛋白中的自主序列编码三折的三级结构信息。这些序列自动折叠成小的连续折叠结构单元集，以在每个单独的框架上生长单独的半折叠。自主序列可以定义为分配给少量连续折叠结构单元的局部序列。它们通过组装疏水区域将疏水区域形成半折叠。半折的特征是通过各自的I型疏水核心网络稳定的离散疏水区域，这些区域存在于每个半折中。半折叠的离散疏水区域通过II型疏水性核心网络中的疏水相互作用通过不同的半折叠的X射线结构观察到，其自身通过不同的半折叠的区域传播。apomyoglobin中半折的最显着特征是，可以通过全珠蛋白的X射线结构对其进行验证，而与自主序列片段特征性折痕的不稳定性无关。这项工作提出了自主序列的第一个描述。
更新日期：2019-01-11
中文翻译：
肌肉红蛋白的X射线结构中出现的肌红蛋白自主序列。
所提出的连续折叠结构单元是分析蛋白质结构的基础。在这里，我们首次使用连续折叠结构单元阐明了肌红蛋白中的两种疏水性核心网络。在肌红蛋白中，清楚地出现了两个自主序列。因此，我们可以在各个半折叠内使用定义明确的疏水性核心网络来表征自主序列。疏水核定义为在不同折叠结构中的一对拓扑局部疏水氨基酸。疏水核的形成对于通过有效的疏水相互作用稳定不同折叠结构必不可少。肌红蛋白中的自主序列编码三折的三级结构信息。这些序列自动折叠成小的连续折叠结构单元集，以在每个单独的框架上生长单独的半折叠。自主序列可以定义为分配给少量连续折叠结构单元的局部序列。它们通过组装疏水区域将疏水区域形成半折叠。半折的特征是通过各自的I型疏水核心网络稳定的离散疏水区域，这些区域存在于每个半折中。半折叠的离散疏水区域通过II型疏水性核心网络中的疏水相互作用通过不同的半折叠的X射线结构观察到，其自身通过不同的半折叠的区域传播。apomyoglobin中半折的最显着特征是，可以通过全珠蛋白的X射线结构对其进行验证，而与自主序列片段特征性折痕的不稳定性无关。这项工作提出了自主序列的第一个描述。
京公网安备 11010802027423号