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Minimally‐invasive Microneedle‐based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid
Electroanalysis ( IF 2.7 ) Pub Date : 2019-01-10 , DOI: 10.1002/elan.201800630
Paolo Bollella 1 , Sanjiv Sharma 2 , Anthony Edward George Cass 3 , Riccarda Antiochia 1
Electroanalysis ( IF 2.7 ) Pub Date : 2019-01-10 , DOI: 10.1002/elan.201800630
Paolo Bollella 1 , Sanjiv Sharma 2 , Anthony Edward George Cass 3 , Riccarda Antiochia 1
Affiliation
Here we report the first mediated pain free microneedle‐based biosensor array for the continuous and simultaneous monitoring of lactate and glucose in artificial interstitial fluid (ISF). The gold surface of the microneedles has been modified by electrodeposition of Au‐multiwalled carbon nanotubes (MWCNTs) and successively by electropolymerization of the redox mediator, methylene blue (MB). Functionalization of the Au‐MWCNTs/polyMB platform with the lactate oxidase (LOX) enzyme (working electrode 1) and with the FAD‐Glucose dehydrogenase (FADGDH) enzyme (working electrode 2) enabled the continuous monitoring of lactate and glucose in the artificial ISF. The lactate biosensor exhibited a high sensitivity (797.4±38.1 μA cm−2 mM−1), a good linear range (10–100 μM) with a detection limit of 3 μM. The performance of the glucose biosensor were also good with a sensitivity of 405.2±24.1 μA cm−2 mM−1, a linear range between 0.05 and 5 mM and a detection limit of 7 μM. The biosensor array was tested to detect the amount of lactate generated after 100 minutes of cycling exercise (12 mM) and of glucose after a normal meal for a healthy patient (10 mM). The results reveal that the new microneedles‐based biosensor array seems to be a promising tool for the development of real‐time wearable devices with a variety of sport medicine and clinical care applications.
中文翻译:
基于微创微针的生物传感器阵列,可同时监测人造组织液中的乳酸和葡萄糖
在这里,我们报告了第一个介导的无痛基于微针的生物传感器阵列,用于连续不断地同时监测人工组织液(ISF)中的乳酸和葡萄糖。微针的金表面已通过电沉积金多壁碳纳米管(MWCNT)进行了修饰,并随后通过氧化还原介体亚甲基蓝(MB)的电聚合进行了修饰。Au-MWCNTs / polyMB平台通过乳酸氧化酶(LOX)酶(工作电极1)和FAD-葡萄糖脱氢酶(FADGDH)酶(工作电极2)的功能实现了对人工ISF中乳酸和葡萄糖的连续监测。乳酸生物传感器显示出高灵敏度(797.4±38.1μAcm -2 mM -1),良好的线性范围(10–100μM),检测极限为3μM。葡萄糖生物传感器的性能也良好,灵敏度为405.2±24.1μAcm -2 mM -1,线性范围为0.05至5mM,检测极限为7μM。测试了生物传感器阵列,以检测出在进行了100分钟的循环运动后产生的乳酸量(12 mM),以及在正常饮食后对于健康患者产生的葡萄糖量(10 mM)。结果表明,基于微针的新型生物传感器阵列似乎是开发具有各种运动医学和临床护理应用的实时可穿戴设备的有前途的工具。
更新日期:2019-01-10
中文翻译:
基于微创微针的生物传感器阵列,可同时监测人造组织液中的乳酸和葡萄糖
在这里,我们报告了第一个介导的无痛基于微针的生物传感器阵列,用于连续不断地同时监测人工组织液(ISF)中的乳酸和葡萄糖。微针的金表面已通过电沉积金多壁碳纳米管(MWCNT)进行了修饰,并随后通过氧化还原介体亚甲基蓝(MB)的电聚合进行了修饰。Au-MWCNTs / polyMB平台通过乳酸氧化酶(LOX)酶(工作电极1)和FAD-葡萄糖脱氢酶(FADGDH)酶(工作电极2)的功能实现了对人工ISF中乳酸和葡萄糖的连续监测。乳酸生物传感器显示出高灵敏度(797.4±38.1μAcm -2 mM -1),良好的线性范围(10–100μM),检测极限为3μM。葡萄糖生物传感器的性能也良好,灵敏度为405.2±24.1μAcm -2 mM -1,线性范围为0.05至5mM,检测极限为7μM。测试了生物传感器阵列,以检测出在进行了100分钟的循环运动后产生的乳酸量(12 mM),以及在正常饮食后对于健康患者产生的葡萄糖量(10 mM)。结果表明,基于微针的新型生物传感器阵列似乎是开发具有各种运动医学和临床护理应用的实时可穿戴设备的有前途的工具。