封面图片显示了一种新的真菌多烯，曲霉烯A1，已从真菌物种构巢曲霉中解锁。已知真菌多烯是由无烯丙基还原酶的高度还原性聚酮化合物合酶（无ER的HR-PKSs）产生的。我们已经确定了一个四基因簇（命名为sdg），该簇编码来自构巢曲霉基因组的无ER的HR-PKS。通过用不同组合的诱导型启动子替换天然启动子并诱导表达，我们成功分离了几种新颖的真菌多烯，称为曲霉。基于鉴定和预测的基因功能的中间体，提出了生物合成。这项工作扩展了真菌多烯生物合成的先前知识，并为将来的基因组挖掘工作铺平了道路。有关更多信息，请参见Y.‐M的交流论文。Chiang C. C. C. Wang等。就在第3期，2019第294页（：10.1002 / cbic.201800486 DOI）。
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Front Cover: Discovery and Elucidation of the Biosynthesis of Aspernidgulenes: Novel Polyenes from Aspergillus Nidulans by Using Serial Promoter Replacement (ChemBioChem 3/2019)
The front cover picture shows a novel fungal polyene, aspernidgulene A1, being unlocked from a fungal species, Aspergillus nidulans. Fungal polyenes are known to be produced by enoyl reductase‐less highly reducing polyketide synthases (ER‐less HR‐PKSs). We have identified a four‐gene cluster (named sdg) that encodes an ER‐less HR‐PKS from the genome of A. nidulans. By replacing the native promoters with inducible promoters in different combinations and inducing expression, we successfully isolated several novel fungal polyenes, named the aspernidgulenes. The biosynthesis was proposed based on the intermediates identified and predicted gene functions. This work extends previous knowledge of fungal polyene biosynthesis and paves the way for future genome‐mining efforts. More information can be found in the communication paper by Y.‐M. Chiang, C. C. C. Wang, et al. on page 329 in Issue 3, 2019 (DOI: 10.1002/cbic.201800486).
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