Noninvasive and Nondestructive Detection of Cowpea Bruchid within Cowpea Seeds with a Hand-Held Raman Spectrometer.,Analytical Chemistry

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Noninvasive and Nondestructive Detection of Cowpea Bruchid within Cowpea Seeds with a Hand-Held Raman Spectrometer.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-01-08 00:00:00 , DOI: 10.1021/acs.analchem.8b05555
Lee Sanchez ₁ , Charles Farber ₁ , Jiaxin Lei ₂ , Keyan Zhu-Salzman ₂ , Dmitry Kurouski _{1,

3}

Affiliation

Insect damage to crops is a serious issue, in particular when the pest dwells within its host. The cowpea bruchid (Callosobruchus maculatus) is an herbivore of legumes including beans and peas. The bruchid lays its eggs on the seeds themselves; after hatching, the larvae burrow into and develop inside the seed, complicating detection and treatment. Left unchecked, two insects could destroy up to 50% of 1 ton of harvest cowpea (Vigna unguiculata) after several months of storage. In this study, we investigated the possibility of using a hand-held Raman spectrometer to detect the pest during its development within intact cowpeas. Our results show that Raman spectroscopy can detect chemical signatures of bruchid larvae as well as their excrements inside the intact seeds. Additionally, using chemometric methods, we distinguished between healthy and infested seeds as well as among seeds hosting developmentally early or late-stage larvae with high accuracy. This study demonstrates Raman spectroscopy’s efficacy in not only detection of pathogens and pests present on the surface of plant leaves and the grain but also inside the seeds. This Raman-based method may prove useful as a rapid means of screening crops for internal pests.

中文翻译：

用手持式拉曼光谱仪无损检测pea豆种子中的Bru豆Bruchid。

虫害对农作物的危害是一个严重的问题，尤其是当虫害驻留在寄主体内时。cow豆bruchid（Callosobruchus maculatus）是豆类和豌豆等豆类的食草动物。杜鹃花将卵产在种子本身上。孵化后，幼虫钻入种子并在种子内部发育，使检测和处理变得复杂。如果任其发展，两只昆虫可能会毁坏1吨收成的pea豆（Vigna unguiculata）的50％）存放几个月后。在这项研究中，我们调查了使用手持式拉曼光谱仪在完整cow豆发育过程中检测有害生物的可能性。我们的结果表明，拉曼光谱可以检测出布鲁氏幼虫的化学特征以及它们在完整种子内的排泄物。此外，使用化学计量学方法，我们可以区分健康种子和受感染种子，还可以准确区分发育中的早期或后期幼虫的种子。这项研究证明了拉曼光谱不仅可以检测植物叶片和谷物表面上的病原体和害虫，而且还可以检测种子内部的病虫害。这种基于拉曼的方法可能被证明是用于筛查农作物内部害虫的快速手段。

更新日期：2019-01-08

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