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Electrochemical Biosensor for DNA Methylation Detection through Hybridization Chain-Amplified Reaction Coupled with a Tetrahedral DNA Nanostructure
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-01-09 00:00:00 , DOI: 10.1021/acsami.8b20144 Xi Chen 1 , Jian Huang 1 , Shu Zhang 1 , Fei Mo , Shasha Su , Yan Li 1 , Lichao Fang 1 , Jun Deng 1 , Hui Huang 1 , Zhaoxun Luo , Junsong Zheng 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-01-09 00:00:00 , DOI: 10.1021/acsami.8b20144 Xi Chen 1 , Jian Huang 1 , Shu Zhang 1 , Fei Mo , Shasha Su , Yan Li 1 , Lichao Fang 1 , Jun Deng 1 , Hui Huang 1 , Zhaoxun Luo , Junsong Zheng 1
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DNA methylation is a key factor in the pathogenesis of gene expression diseases or malignancies. Thus, it has become a significant biomarker for the diagnosis and prognosis of these diseases. In this paper, we designed an ultrasensitive and specific electrochemical biosensor for DNA methylation detection. The platform consisted of stem–loop–tetrahedron composite DNA probes anchoring at a Au nanoparticle-coated gold electrode, a restriction enzyme digestion of HpaII, and signal amplification procedures including electrodeposition of Au nanoparticles, hybridization chain reaction, and horseradish peroxidase enzymatic catalysis. Under optimal conditions, the design showed a broad dynamic range from 1 aM to 1 pM and a detection limit of about 0.93 aM. The approach also showed ideal specificity, repeatability, and stability. The recovery test demonstrated that the design is a promising platform for DNA methylation detection under clinical circumstances and could meet the need for cancer diagnosis.
中文翻译：
电化学生物传感器通过四链DNA纳米结构的杂交链扩增反应与DNA甲基化检测
DNA甲基化是基因表达疾病或恶性肿瘤发病机理中的关键因素。因此，它已成为这些疾病的诊断和预后的重要生物标志物。在本文中，我们设计了一种用于DNA甲基化检测的超灵敏且特异的电化学生物传感器。该平台由锚固在Au纳米涂层金电极上的茎-环-四面体复合DNA探针组成，Hpa的限制性内切酶消化II，信号放大程序包括Au纳米粒子的电沉积，杂交链反应和辣根过氧化物酶的酶催化。在最佳条件下，设计显示了从1 aM到1 pM的宽动态范围，检测极限约为0.93 aM。该方法还显示出理想的特异性，可重复性和稳定性。恢复测试表明，该设计在临床情况下是用于DNA甲基化检测的有前途的平台，可以满足癌症诊断的需求。
更新日期：2019-01-09
中文翻译：
电化学生物传感器通过四链DNA纳米结构的杂交链扩增反应与DNA甲基化检测
DNA甲基化是基因表达疾病或恶性肿瘤发病机理中的关键因素。因此，它已成为这些疾病的诊断和预后的重要生物标志物。在本文中，我们设计了一种用于DNA甲基化检测的超灵敏且特异的电化学生物传感器。该平台由锚固在Au纳米涂层金电极上的茎-环-四面体复合DNA探针组成，Hpa的限制性内切酶消化II，信号放大程序包括Au纳米粒子的电沉积，杂交链反应和辣根过氧化物酶的酶催化。在最佳条件下，设计显示了从1 aM到1 pM的宽动态范围，检测极限约为0.93 aM。该方法还显示出理想的特异性，可重复性和稳定性。恢复测试表明，该设计在临床情况下是用于DNA甲基化检测的有前途的平台，可以满足癌症诊断的需求。
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