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The ecdysone receptor complex is essential for the reproductive success in the female desert locust, Schistocerca gregaria.
Scientific Reports ( IF 3.8 ) Pub Date : 2019-01-09 , DOI: 10.1038/s41598-018-36763-9
Cynthia Lenaerts 1 , Elisabeth Marchal 1 , Paulien Peeters 1 , Jozef Vanden Broeck 1
Affiliation  

Ecdysteroid hormones influence the development and reproduction of arthropods by binding a heterodimeric complex of nuclear receptors, the ecdysone receptor (EcR) and the retinoid-X-receptor/ultraspiracle (RXR/USP). Here, we report on the in vivo role(s) of the ecdysone receptor complex, SchgrEcR/SchgrRXR, in the female reproductive physiology of a major phytophagous pest insect, i.e. the desert locust, Schistocerca gregaria. Tissue and temporal distribution profiles were analysed during the first gonadotrophic cycle of adult female locusts. RNA interference was used as a reverse genetics tool to investigate the in vivo role of the ecdysone receptor complex in ovarian maturation, oogenesis, fertility and fecundity. We discovered that silencing the ecdysone receptor complex in S. gregaria resulted in impaired ovulation and oviposition, indicative for a crucial role of this complex in chorion formation. We also found evidence for a feedback of SchgrEcR/SchgrRXR on juvenile hormone biosynthesis by the corpora allata. Furthermore, we observed a tissue-dependent effect of the SchgrEcR/SchgrRXR knockdown on the transcript levels of the insulin receptor and neuroparsin 3 and 4. The insulin receptor transcript levels were upregulated in the brain, but not the fat body and gonads. Neuroparsins 3 and 4 transcript levels were down regulated in the brain and fat body, but not in the gonads.

中文翻译:

蜕皮激素受体复合物对于雌性沙漠蝗Schistocerca gregaria的繁殖成功至关重要。

蜕皮甾类激素通过结合核受体,蜕皮激素受体(EcR)和类维生素X受体/超螺旋体(RXR / USP)的异二聚体复合物来影响节肢动物的发育和繁殖。在这里,我们报告蜕皮激素受体复合物SchgrEcR / SchgrRXR在主要植物吞噬性害虫,即沙漠蝗虫,日本血吸虫中的雌性生殖生理学中的体内作用。在成年雌性蝗虫的第一个性腺生殖周期中分析了组织和时间分布特征。RNA干扰被用作反向遗传学工具,以研究蜕皮激素受体复合物在卵巢成熟,卵子发生,繁殖力和生殖力方面的体内作用。我们发现沉默沙丁球菌中的蜕皮激素受体复合物会导致排卵和排卵障碍,表明该复合物在绒毛膜形成中的关键作用。我们还找到了证据证明SchgrEcR / SchgrRXR对全集的幼体激素生物合成的反馈。此外,我们观察到了SchgrEcR / SchgrRXR组合式对胰岛素受体和神经parsin 3和4的转录水平有组织依赖性的作用。胰岛素受体的转录水平在大脑中被上调,但在脂肪体和性腺中却不被上调。在大脑和脂肪体内,神经parsins 3和4的转录水平被下调,但在性腺中却没有。我们观察到了SchgrEcR / SchgrRXR组合的组织依赖性效应对胰岛素受体和神经parsin 3和4的转录水平的影响。胰岛素受体的转录水平在大脑中被上调,但在脂肪体和性腺中却不被上调。在大脑和脂肪体内,神经parsins 3和4的转录水平被下调,但在性腺中却没有。我们观察到了SchgrEcR / SchgrRXR组合的组织依赖性效应对胰岛素受体和神经parsin 3和4的转录水平的影响。胰岛素受体的转录水平在大脑中被上调,但在脂肪体和性腺中却不被上调。在大脑和脂肪体内,神经parsins 3和4的转录水平被下调,但在性腺中却没有。
更新日期:2019-01-09
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