明胶/ PCL–PEG–PCL的纳米复合水凝胶支架的制备及体外评价，用于软骨组织工程,ACS Omega

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明胶/ PCL–PEG–PCL的纳米复合水凝胶支架的制备及体外评价，用于软骨组织工程
ACS Omega ( IF 3.7 ) Pub Date : 2019-01-07 00:00:00 , DOI: 10.1021/acsomega.8b02593
Nahideh Asadi , Effat Alizadeh , Azizeh Rahmani Del Bakhshayesh , Ebrahim Mostafavi ₁ , Abolfazl Akbarzadeh , Soodabeh Davaran

Affiliation

软骨组织的自我修复受到限制。具有3D水合聚合物的水凝胶具有使其成为软骨组织工程的合适材料的特性。在这项研究中，一种新型的纳米复合材料的水凝胶基于明胶/聚己内酯-聚乙二醇（凝胶/ PCEC-TGFβ ₁制备）并评估衍生自脂肪组织（h-AD-MSC）的人间充质干细胞的增殖和软骨分化。通过扫描电子显微镜（SEM）表征所制备的支架的孔隙率。评价了所制备的水凝胶的溶胀行为和机械性能。将h-AD-MSC在每个支架上培养14天，并通过SEM研究细胞附着。使用MTT测定法研究细胞活力。进行甲苯胺蓝染色以评估蛋白聚糖的合成。实时PCR测定法用于监测胶原蛋白II和聚集蛋白聚糖基因的表达。数据显示，与对照组相比，支架支持细胞增殖。甲苯胺蓝染色的组织学分析显示糖胺聚糖（GAGs）的沉积。实时PCR分析还表明，水凝胶，尤其是纳米复合水凝胶，具有表达胶原蛋白II和聚集蛋白聚糖基因的潜力。因此，Gel / PCEC–TGFβ₁纳米复合水凝胶具有h-AD-MSCs的生长和分化潜能，并且可能是用于软骨组织工程的有前途的生物材料。

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Fabrication and in Vitro Evaluation of Nanocomposite Hydrogel Scaffolds Based on Gelatin/PCL–PEG–PCL for Cartilage Tissue Engineering

The self-repair of cartilage tissue is limited. Hydrogels with 3D hydrated polymers have properties that make them a suitable material for cartilage tissue engineering. In this study, a novel nanocomposite hydrogel based on gelatin/polycaprolactone–polyethylene glycol (Gel/PCEC–TGFβ₁) was prepared and evaluated for the proliferation and chondrogenic differentiation of human mesenchymal stem cells derived from adipose tissue (h-AD-MSCs). The porosity of the prepared scaffolds was characterized by scanning electron microscopy (SEM). Swelling behavior and mechanical properties of the prepared hydrogels were evaluated. h-AD-MSCs were cultured on each scaffold for 14 days, and cell attachment was investigated by SEM. The cell viability was studied using MTT assay. Toluidine blue staining was carried out to evaluate the synthesis of proteoglycan. Real-time PCR assay was used to monitor the expression of collagen II and aggrecan genes. The data showed that scaffolds support cell proliferation in comparison to control group. Histological analyses with toluidine blue staining exhibited the deposition of glycosaminoglycans (GAGs). Real-time PCR analysis also indicated that hydrogels, especially the nanocomposite hydrogel, showed the potential to express collagen II and aggrecan genes. Therefore, the Gel/PCEC–TGFβ₁ nanocomposite hydrogels have the potential for the growth and differentiation of h-AD-MSCs and can be a promising biomaterial for cartilage tissue engineering.

更新日期：2019-01-07

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