Plasmonic Colloidosome-Based Single Cell Detector: A Strategy for Individual Cell Secretion Sensing.,Analytical Chemistry

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Plasmonic Colloidosome-Based Single Cell Detector: A Strategy for Individual Cell Secretion Sensing.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-01-04 00:00:00 , DOI: 10.1021/acs.analchem.8b04850
Xinjun Wang ₁ , Ji Ji ₁ , Tingting Liu ₂ , Yujie Liu ₁ , Liang Qiao ₁ , Baohong Liu ₁

Affiliation

Phenotyping single cells based on molecules that they secrete or consume is a key bottleneck in many biotechnology applications. Here, we demonstrate a new approach for detecting secretions from individual single cells, namely, plasmonic colloidosome-based single cell detector (PCSD). This strategy uses colloidosomes to encapsulate single cells and aid molecular detection. Colloidosomes are constructed by emulsifying lactate probe and hydrophobic ligand dual-functionalized silver nanoparticles on an immiscible liquid–liquid interface. The established colloidosome-based platform exhibits negligible surface-enhanced Raman scattering (SERS) background interference, ultrasensitive SERS response, and excellent signal reproducibility in response to acidification of the medium inside the colloidosomes. Taking lactate as a model molecule, the acidification induced by single cells confined in the colloidosomes is detected. The approach shows promising applicability in single cell analysis based on extracellular metabolites production or consumption.

中文翻译：

基于等离子胶体的单细胞检测器：单个细胞分泌感知的策略。

基于单个细胞分泌或消耗的分子进行表型鉴定是许多生物技术应用中的关键瓶颈。在这里，我们演示了一种检测单个细胞分泌物的新方法，即基于等离子胶体的单细胞检测器（PCSD）。该策略使用胶体包裹单个细胞并帮助分子检测。胶体是通过在不混溶的液-液界面上乳化乳酸探针和疏水性配体双功能化的银纳米颗粒而构建的。建立的基于胶体的平台表现出微不足道的表面增强拉曼散射（SERS）背景干扰，超灵敏SERS响应以及对胶体内部介质酸化的出色信号再现性。以乳酸为模型分子，检测由封闭在胶体中的单细胞诱导的酸化。该方法在基于细胞外代谢物产生或消耗的单细胞分析中显示出广阔的应用前景。

更新日期：2019-01-04

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