Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants.,Nature Communications

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Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants.
Nature Communications ( IF 14.7 ) Pub Date : 2019-01-03 , DOI: 10.1038/s41467-018-07969-2
Yichun Qian ₁ , Joseph H Lynch ₂ , Longyun Guo ₂ , David Rhodes ₁ , John A Morgan _{2,

3} , Natalia Dudareva _{1,

2,

4}

Affiliation

In addition to being a vital component of proteins, phenylalanine is also a precursor of numerous aromatic primary and secondary metabolites with broad physiological functions. In plants phenylalanine is synthesized predominantly via the arogenate pathway in plastids. Here, we describe the structure, molecular players and subcellular localization of a microbial-like phenylpyruvate pathway for phenylalanine biosynthesis in plants. Using a reverse genetic approach and metabolic flux analysis, we provide evidence that the cytosolic chorismate mutase is responsible for directing carbon flux towards cytosolic phenylalanine production via the phenylpyruvate pathway. We also show that an alternative transcription start site of a known plastidial enzyme produces a functional cytosolic prephenate dehydratase that catalyzes the conversion of prephenate to phenylpyruvate, the intermediate step between chorismate mutase and phenylpyruvate aminotransferase. Thus, our results complete elucidation of phenylalanine biosynthesis via phenylpyruvate in plants, showing that this pathway splits from the known plastidial arogenate pathway at chorismate, instead of prephenate as previously thought, and the complete pathway is localized in the cytosol.

中文翻译：

植物中胞质胆汁酸后苯丙氨酸生物合成途径的完成。

苯丙氨酸不仅是蛋白质的重要组成部分，还是众多具有广泛生理功能的芳香族一级和二级代谢产物的前体。在植物中，苯丙氨酸主要通过质体中的Arogenate途径合成。在这里，我们描述了植物中苯丙氨酸生物合成的微生物样苯丙酮酸途径的结构，分子分子和亚细胞定位。使用反向遗传方法和代谢通量分析，我们提供了证据，表明胞质分支氨酸突变酶负责通过苯丙酮酸途径将碳通量导向胞质苯丙氨酸生产。我们还显示，已知质体酶的替代转录起始位点可产生功能性胞质酚醛酸脱水酶，该酶催化酚醛向苯基丙酮酸的转化，即分支酸酯突变酶和苯基丙酮酸氨基转移酶之间的中间步骤。因此，我们的结果完全阐明了通过植物丙酮酸苯酯实现的苯丙氨酸的生物合成，表明该途径与分支酸中已知的质构Arogenate途径分离，而不是像以前所想的那样是苯甲酸酯，并且完整的途径位于胞质溶胶中。

更新日期：2019-01-03

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