Super-resolution microscopy demystified.,Nature Cell Biology

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Super-resolution microscopy demystified.
Nature Cell Biology ( IF 17.3 ) Pub Date : 2019-01-02 , DOI: 10.1038/s41556-018-0251-8
Lothar Schermelleh ₁ , Alexia Ferrand ₂ , Thomas Huser ₃ , Christian Eggeling _{4,

5} , Markus Sauer ₆ , Oliver Biehlmaier ₂ , Gregor P C Drummen _{7,

8}

Affiliation

Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that restricts the optical resolution to roughly 250 nm and was previously thought to be impenetrable. SRM techniques allow the visualization of subcellular organization with unprecedented detail, but also confront biologists with the challenge of selecting the best-suited approach for their particular research question. Here, we provide guidance on how to use SRM techniques advantageously for investigating cellular structures and dynamics to promote new discoveries.

中文翻译：

超分辨率显微镜揭秘。

超分辨率显微镜 (SRM) 绕过了衍射极限，这是一种将光学分辨率限制在大约 250 nm 的物理屏障，以前被认为是不可逾越的。 SRM 技术可以以前所未有的细节可视化亚细胞组织，但也让生物学家面临着为他们的特定研究问题选择最适合的方法的挑战。在这里，我们提供有关如何有利地使用 SRM 技术来研究细胞结构和动力学以促进新发现的指导。

更新日期：2019-01-25

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