Firefly Luciferase Mutants Allow Substrate‐Selective Bioluminescence Imaging in the Mouse Brain,Angewandte Chemie International Edition

当前位置： X-MOL 学术 › Angew. Chem. Int. Ed. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Firefly Luciferase Mutants Allow Substrate‐Selective Bioluminescence Imaging in the Mouse Brain
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2016-03-17 , DOI: 10.1002/anie.201511350
Spencer T. Adams ₁ , David M. Mofford ₁ , G. S. Kiran Kumar Reddy ₁ , Stephen C. Miller ₁

Affiliation

Bioluminescence imaging is a powerful approach for visualizing specific events occurring inside live mice. Animals can be made to glow in response to the expression of a gene, the activity of an enzyme, or the growth of a tumor. But bioluminescence requires the interaction of a luciferase enzyme with a small‐molecule luciferin, and its scope has been limited by the mere handful of natural combinations. Herein, we show that mutants of firefly luciferase can discriminate between natural and synthetic substrates in the brains of live mice. When using adeno‐associated viral (AAV) vectors to express luciferases in the brain, we found that mutant luciferases that are inactive or weakly active with d‐luciferin can light up brightly when treated with the aminoluciferins CycLuc1 and CycLuc2 or their respective FAAH‐sensitive luciferin amides. Further development of selective luciferases promises to expand the power of bioluminescence and allow multiple events to be imaged in the same live animal.

中文翻译：

萤火虫萤光素酶突变体可在小鼠大脑中进行底物选择性生物发光成像

生物发光成像是一种可视化活小鼠内部发生的特定事件的强大方法。可以使动物发光，以响应基因的表达，酶的活性或肿瘤的生长。但是生物发光需要萤光素酶与小分子萤光素的相互作用，其范围仅受少数几种自然组合的限制。在这里，我们显示萤火虫荧光素酶的突变体可以区分活小鼠大脑中的天然和合成底物。当使用腺相关病毒（AAV）载体在大脑中表达萤光素酶时，我们发现与d无活性或弱活性的突变型萤光素酶当用氨基荧光素CycLuc1和CycLuc2或它们各自的FAAH敏感的荧光素酰胺处理时，荧光素可以明亮地发光。选择性荧光素酶的进一步发展有望扩大生物发光的能力，并允许在同一只活动物中对多个事件进行成像。

更新日期：2016-03-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南