Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii.,Scientific Reports

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Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii.
Scientific Reports ( IF 3.8 ) Pub Date : 2018-12-21 , DOI: 10.1038/s41598-018-36732-2
Shin Ohsawa ₁ , Susumu Nishida ₁ , Masahide Oku ₁ , Yasuyoshi Sakai _{1,

2} , Hiroya Yurimoto ₁

Affiliation

In methylotrophic yeasts, the expression of methanol-inducible genes is repressed by ethanol even in the presence of methanol, a phenomenon called ethanol repression. The mechanism of ethanol repression in Komagataella phaffii (Pichia pastoris) was studied, and acetyl-CoA synthesis from ethanol by sequential reactions of alcohol dehydrogenase, aldehyde dehydrogenase and acetyl-CoA synthetase (ACS) was involved in ethanol repression. Molecular analysis of the ACS-encoding gene product KpAcs1 revealed that its N-terminal motif, which is conserved in methylotrophic yeasts, was required for ethanol repression. ACS activity was downregulated during methanol-induced gene expression, which partially depended on autophagy. In addition, acetyl-CoA synthesis and phosphorylation of a transcription factor KpMxr1 were found to contribute to ethanol repression in a synergistic manner.

中文翻译：

乙醇可通过乙酰辅酶A合成抑制酵母Komagataella phaffii中甲醇诱导型基因的表达。

在甲基营养型酵母中，即使在存在甲醇的情况下，乙醇也会抑制甲醇可诱导基因的表达，这种现象称为乙醇抑制。研究了噬菌体（Piaia pastoris）的乙醇抑制机理，并通过乙醇脱氢酶，醛脱氢酶和乙酰辅酶A合成酶（ACS）的顺序反应由乙醇合成乙酰辅酶A。ACS编码基因产物KpAcs1的分子分析显示，其N端基序（在甲基营养型酵母中保守）是抑制乙醇的必需条件。在甲醇诱导的基因表达过程中，ACS活性被下调，这部分取决于自噬。此外，

更新日期：2018-12-21

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