Staining Traditional Colloidal Gold Test Strips with Pt Nanoshell Enables Quantitative Point-of-Care Testing with Simple and Portable Pressure Meter Readout,ACS Applied Materials & Interfaces

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Staining Traditional Colloidal Gold Test Strips with Pt Nanoshell Enables Quantitative Point-of-Care Testing with Simple and Portable Pressure Meter Readout
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-12-20 00:00:00 , DOI: 10.1021/acsami.8b15562
Di Huang ₁ , Bingqian Lin ₁ , Yanling Song ₂ , Zhichao Guan ₃ , Jie Cheng ₁ , Zhi Zhu ₁ , Chaoyong Yang _{1,

2}

Affiliation

Traditional immunochromatographic test strips based on colloidal gold are effective devices for portable and low-cost point-of-care (POC) testing. Nevertheless, they still suffer from the limitation of qualitative or semiquantitative tests via naked-eye detection. Replacement of gold with other signal entities, such as magnetic particles or fluorescent particles, requires professional instrumentation to obtain quantitative results. A pressure-based assay with platinum nanoparticles (PtNPs) can provide quantitative results using a portable pressure meter but is also hampered by the long-term instability of PtNPs. Consequently, we developed a Pt-staining method based on test strips to create platinum nanoshells on the surface of colloidal gold. This method not only preserves the original advantages of colloidal gold with easy synthesis and decoration but also introduces PtNPs with excellent catalytic activity as signal labels to achieve sensitive quantitative detection. Myoglobin was tested as a model target, and the limit of detection was 5.47 ng/mL in 20% diluted serum samples, which satisfies the requirements for clinical monitoring of acute myocardial infarction. In addition, the two most common colloidal gold strips available in the marketplace were applied to demonstrate the compatibility of Pt-staining. Taking advantage of low cost, user-friendliness, compatibility, simplicity, and stability, colloidal gold test strips with Pt-staining are expected to satisfy the need for quantitative POC testing of biomarkers, especially in resource-limited regions.

中文翻译：

使用Pt纳米壳对传统的胶体金测试条进行染色，可通过简单且便携式的压力表读数进行定量的现场护理测试

基于胶体金的传统免疫色谱测试条是便携式和低成本即时护理（POC）测试的有效设备。然而，他们仍然遭受通过肉眼检测进行的定性或半定量测试的局限。用其他信号实体（例如磁性颗粒或荧光颗粒）代替金，需要专业的仪器才能获得定量结果。铂纳米颗粒（PtNPs）的基于压力的测定可以使用便携式压力计提供定量结果，但也受PtNPs长期不稳定性的困扰。因此，我们开发了一种基于测试条的Pt染色方法，以在胶体金的表面上创建铂纳米壳。该方法不仅保留了胶体金的原始优势，易于合成和修饰，而且还引入了具有出色催化活性的PtNPs作为信号标记，从而实现了灵敏的定量检测。将肌红蛋白作为模型目标进行测试，在20％稀释的血清样品中的检出限为5.47 ng / mL，符合临床上急性心肌梗塞的监测要求。另外，使用市场上可用的两种最常见的胶体金带来证明Pt染色的兼容性。利用低成本，用户友好性，兼容性，简单性和稳定性的优势，带有Pt染色的胶体金试纸有望满足对生物标志物进行定量POC测试的需求，尤其是在资源有限的地区。

更新日期：2018-12-20

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