Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis,Journal of Agricultural and Food Chemistry

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Increased Glucosinolate Production in Brassica oleracea var. italica Cell Cultures Due to Coronatine Activated Genes Involved in Glucosinolate Biosynthesis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-19 00:00:00 , DOI: 10.1021/acs.jafc.8b04298
P. J. Sánchez-Pujante ₁ , A. B. Sabater-Jara ₁ , S. Belchí-Navarro ₁ , M. A. Pedreño ₁ , L. Almagro ₁

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In this work, the effect of different elicitors and culture conditions on the production of glucosinolates in broccoli cell cultures was studied. The results showed that 0.5 μM coronatine was the best elicitor for increasing glucosinolate production (205-fold increase over untreated cells after 72 h of treatment). Furthermore, the expression levels of some genes related to the biosynthetic pathway of glucosinolates as well as three Myb transcription factors also have been studied. The highest glucosinolate levels found in coronatine-treated cells were closely correlated with the highest gene expression levels of Cyp79b2, Cyp83b1, St5a, Myb51, and Myb122 after 6 h of treatment. The data shown in this study provide new insight into the key metabolic steps involved in the biosynthesis of glucosinolates, which will be of use for future applications of metabolic engineering techniques in broccoli.

中文翻译：

甘蓝变种中芥子油苷产量的增加。涉及芥子油苷生物合成的冠状花序激活基因引起的italica细胞培养

在这项工作中，研究了不同引发剂和培养条件对西兰花细胞培养物中芥子油苷产生的影响。结果表明，0.5μM的冠状动脉素是增加芥子油苷产量的最佳引发剂（处理72小时后，其含量是未处理细胞的205倍）。此外，还研究了与芥子油苷的生物合成途径有关的一些基因的表达水平以及三种Myb转录因子。在冠状动脉处理的细胞中发现的最高芥子油苷水平与Cyp79b2，Cyp83b1，St5a，Myb51和Myb122的最高基因表达水平密切相关治疗6小时后。这项研究中显示的数据为芥子油苷生物合成中涉及的关键代谢步骤提供了新的见识，这些代谢步骤将被用于花椰菜中的代谢工程技术的未来应用。

更新日期：2018-12-19

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