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Paper Electrode-Based Flexible Pressure Sensor for Point-of-Care Immunoassay with Digital Multimeter.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-12-20 00:00:00 , DOI: 10.1021/acs.analchem.8b04635 Zhenzhong Yu 1 , Yun Tang 2 , Guoneng Cai 1 , Rongrong Ren 1 , Dianping Tang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-12-20 00:00:00 , DOI: 10.1021/acs.analchem.8b04635 Zhenzhong Yu 1 , Yun Tang 2 , Guoneng Cai 1 , Rongrong Ren 1 , Dianping Tang 1
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A novel paper electrode-based flexible pressure sensor modified with multiwalled carbon nanotubes was designed for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA) with digital multimeter readout. The portable POC testing device consisted of flexible pressure sensor equipped with a paper electrode and connected through syringe tubing to a single-break microplate. The immunoreaction was initially carried out on the microplate with a sandwich-type assay format using platinum nanozyme-labeled secondary antibody for the gas generation. Upon addition of hydrogen peroxide (H2O2), platinum nanozyme (catalase-like mimic) reduced it into hydrogen oxide and oxygen (O2). The overflowing oxygen gas increased the pressure of the multiwalled carbon nanotube-functionalized paper electrode in a homemade pressure-tight system, and the increased pressure could be readily monitored using the paper electrode-based flexible pressure-tight sensor with a digital multimeter readout. The detectable signal mainly derived from the resistance change of pressure sensor because of its deformation with the assistance of the as-generated gas, and the shift in the resistance could be allowed to detect the gas pressure even as low as 80 Pa. Under optimum conditions, pressure sensor-based immunoassay exhibited good resistance responses toward target CEA within a linear range of 0.5–60 ng/mL at a detection limit of 167 pg/mL. Moreover, our strategy provided acceptable reproducibility, precision, high specificity, and good accordance with the commercial CEA ELISA kit for detecting human serum specimens.
中文翻译：
基于纸电极的柔性压力传感器，用于带有数字万用表的现场护理免疫分析。
设计了一种新颖的基于纸电极的柔性压力传感器，该传感器采用多壁碳纳米管修饰，用于通过数字万用表读数对癌胚抗原（CEA）进行即时护理（POC）免疫测定。便携式POC测试设备由柔性压力传感器组成，该压力传感器配有纸电极，并通过注射器管连接到单断点微孔板。免疫反应首先在三明治板上用三明治型测定法在微板上进行，使用铂纳米酶标记的第二抗体进行产气。加入过氧化氢（H 2 O 2）后，铂纳米酶（过氧化氢酶样模拟物）将其还原为氧化氢和氧气（O 2））。氧气的溢出增加了自制压力密封系统中多壁碳纳米管功能化纸电极的压力，并且使用带数字万用表读数的基于纸电极的柔性压力传感器可轻松监测升高的压力。可检测信号主要来自压力传感器的电阻变化，这是由于其在产生的气体的帮助下变形而引起的，并且即使在低至80 Pa的压力下，也可以通过电阻的变化来检测气体压力。在最佳条件下，基于压力传感器的免疫测定在0.5–60 ng / mL的线性范围内，对目标CEA表现出良好的耐药性，检出限为167 pg / mL。此外，我们的策略还提供了可接受的可重复性，精确度，高特异性，
更新日期：2018-12-20
中文翻译：
基于纸电极的柔性压力传感器，用于带有数字万用表的现场护理免疫分析。
设计了一种新颖的基于纸电极的柔性压力传感器，该传感器采用多壁碳纳米管修饰，用于通过数字万用表读数对癌胚抗原（CEA）进行即时护理（POC）免疫测定。便携式POC测试设备由柔性压力传感器组成，该压力传感器配有纸电极，并通过注射器管连接到单断点微孔板。免疫反应首先在三明治板上用三明治型测定法在微板上进行，使用铂纳米酶标记的第二抗体进行产气。加入过氧化氢（H 2 O 2）后，铂纳米酶（过氧化氢酶样模拟物）将其还原为氧化氢和氧气（O 2））。氧气的溢出增加了自制压力密封系统中多壁碳纳米管功能化纸电极的压力，并且使用带数字万用表读数的基于纸电极的柔性压力传感器可轻松监测升高的压力。可检测信号主要来自压力传感器的电阻变化，这是由于其在产生的气体的帮助下变形而引起的，并且即使在低至80 Pa的压力下，也可以通过电阻的变化来检测气体压力。在最佳条件下，基于压力传感器的免疫测定在0.5–60 ng / mL的线性范围内，对目标CEA表现出良好的耐药性，检出限为167 pg / mL。此外，我们的策略还提供了可接受的可重复性，精确度，高特异性，
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