Triarylmethane Fluorophores Resistant to Oxidative Photobluing,Journal of the American Chemical Society

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Triarylmethane Fluorophores Resistant to Oxidative Photobluing
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2018-12-18 , DOI: 10.1021/jacs.8b11036
Alexey N. Butkevich _{1,

2} , Mariano L. Bossi ₂ , Gražvydas Lukinavičius ₁ , Stefan W. Hell _{1,

2}

Affiliation

Spectral stability of small-molecule fluorescent probes is required for correct interpretation and reproducibility of multicolor fluorescence imaging data, in particular under high (de)excitation light intensities of super-resolution imaging or in single-molecule applications. We propose a synthetic approach to a series of spectrally stable rhodamine fluorophores based on sequential Ru- and Cu-catalyzed transformations, evaluate their stability against photobleaching and photoconversion in the context of other fluorophores using chemometric analysis, and demonstrate chemical reactivity of fluorophore photoproducts. The substitution patterns providing the photoconversion-resistant triarylmethane fluorophores have been identified, and the applicability of nonbluing labels in live-cell STED nanoscopy is demonstrated.

中文翻译：

抗氧化光蓝的三芳基甲烷荧光团

多色荧光成像数据的正确解释和再现需要小分子荧光探针的光谱稳定性，特别是在超分辨率成像的高（去）激发光强度下或在单分子应用中。我们提出了一种基于连续 Ru 和 Cu 催化转化的一系列光谱稳定罗丹明荧光团的合成方法，使用化学计量分析在其他荧光团的背景下评估它们对光漂白和光转换的稳定性，并证明了荧光团光产物的化学反应性。已经确定了提供抗光转换三芳基甲烷荧光团的取代模式，并证明了非蓝色标记在活细胞 STED 纳米显微镜中的适用性。

更新日期：2018-12-18

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