当前位置:
X-MOL 学术
›
Commun. Biol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Global transcriptome analysis of the aphelid Paraphelidium tribonemae supports the phagotrophic origin of fungi.
Communications Biology ( IF 5.2 ) Pub Date : 2018-12-19 , DOI: 10.1038/s42003-018-0235-z Guifré Torruella 1 , Xavier Grau-Bové 2 , David Moreira 1 , Sergey A Karpov 1, 3 , John A Burns 4 , Arnau Sebé-Pedrós 5 , Eckhard Völcker 6 , Purificación López-García 1
Communications Biology ( IF 5.2 ) Pub Date : 2018-12-19 , DOI: 10.1038/s42003-018-0235-z Guifré Torruella 1 , Xavier Grau-Bové 2 , David Moreira 1 , Sergey A Karpov 1, 3 , John A Burns 4 , Arnau Sebé-Pedrós 5 , Eckhard Völcker 6 , Purificación López-García 1
Affiliation
Aphelids are little-known phagotrophic parasites of algae whose life cycle and morphology resemble those of the parasitic rozellids (Cryptomycota, Rozellomycota). In previous phylogenetic analyses of RNA polymerase and rRNA genes, aphelids, rozellids and Microsporidia (parasites of animals) formed a clade, named Opisthosporidia, which appeared as the sister group to Fungi. However, the statistical support for the Opisthosporidia was always moderate. Here, we generated full life-cycle transcriptome data for the aphelid species Paraphelidium tribonemae. In-depth multi-gene phylogenomic analyses using several protein datasets place this aphelid as the closest relative of fungi to the exclusion of rozellids and Microsporidia. In contrast with the comparatively reduced Rozella allomycis genome, we infer a rich, free-living-like aphelid proteome, with a metabolism similar to fungi, including cellulases likely involved in algal cell-wall penetration and enzymes involved in chitin biosynthesis. Our results suggest that fungi evolved from complex aphelid-like ancestors that lost phagotrophy and became osmotrophic.
中文翻译:
蚜虫 Paraphelidium tribonemae 的整体转录组分析支持真菌的吞噬起源。
蚜虫是鲜为人知的藻类吞噬寄生虫,其生命周期和形态与寄生罗泽菌门(隐菌门、Rozellomycota)相似。在之前对RNA聚合酶和rRNA基因的系统发育分析中,蚜虫、rozellids和微孢子虫(动物寄生虫)形成了一个分支,称为后孢子虫,它是真菌的姐妹群。然而,对后孢子虫的统计支持始终是中等的。在这里,我们生成了蚜虫物种 Paraphelidium tribonemae 的完整生命周期转录组数据。使用多个蛋白质数据集进行的深入的多基因系统发育分析表明,除了罗泽尔科动物和微孢子虫之外,这种蚜虫是真菌的最接近的亲戚。与相对减少的Rozella allomycis基因组相比,我们推断出丰富的、类似自由生活的蚜虫蛋白质组,其代谢类似于真菌,包括可能参与藻类细胞壁渗透的纤维素酶和参与几丁质生物合成的酶。我们的结果表明,真菌是从复杂的类蚜虫祖先进化而来的,它们失去了吞噬能力并变得渗透营养。
更新日期:2019-01-26
中文翻译:
蚜虫 Paraphelidium tribonemae 的整体转录组分析支持真菌的吞噬起源。
蚜虫是鲜为人知的藻类吞噬寄生虫,其生命周期和形态与寄生罗泽菌门(隐菌门、Rozellomycota)相似。在之前对RNA聚合酶和rRNA基因的系统发育分析中,蚜虫、rozellids和微孢子虫(动物寄生虫)形成了一个分支,称为后孢子虫,它是真菌的姐妹群。然而,对后孢子虫的统计支持始终是中等的。在这里,我们生成了蚜虫物种 Paraphelidium tribonemae 的完整生命周期转录组数据。使用多个蛋白质数据集进行的深入的多基因系统发育分析表明,除了罗泽尔科动物和微孢子虫之外,这种蚜虫是真菌的最接近的亲戚。与相对减少的Rozella allomycis基因组相比,我们推断出丰富的、类似自由生活的蚜虫蛋白质组,其代谢类似于真菌,包括可能参与藻类细胞壁渗透的纤维素酶和参与几丁质生物合成的酶。我们的结果表明,真菌是从复杂的类蚜虫祖先进化而来的,它们失去了吞噬能力并变得渗透营养。