Hydrogen production by immobilized Enterobacter aerogenes on functionalized multi-walled carbon nanotube (MWCNT-COOH) in repeated batch mode was studied. Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FESEM) were employed to confirm immobilization of E. aerogenes successfully. The effect of MWCNT-COOH concentrations (0.2, 0.6, and 1.2 mg/mL) on hydrogen production was investigated. The present study showed that immobilized E. aerogenes on 1.2 mg/mL MWCNT-COOH resulted in higher hydrogen yield (2.2 moL/mol glucose), hydrogen production rate (2.72 L/L.h), and glucose degradation efficiency (96.20%) and shorter the lag phase (1 h) compared to the free E. aerogenes. Modified Gompertz and Logistic models were employed to predict the cumulative hydrogen production successfully.

研究了功能化的多壁碳纳米管（MWCNT-COOH）上以重复批处理方式固定化的产气肠杆菌产生的氢。傅里叶变换红外（FTIR）光谱和场发射扫描电子显微镜（FESEM）用于成功地确定了产气大肠杆菌的固定化。研究了MWCNT-COOH浓度（0.2、0.6和1.2 mg / mL）对产氢的影响。本研究表明，固定化产气肠杆菌上1.2毫克/毫升MWCNT-COOH导致更高的产氢量（2.2摩尔/摩尔葡萄糖），产氢速率（2.72 L / LH），和葡萄糖降解效率（96.20％）和更短的迟滞期（1小时）与游离的产气大肠杆菌相比。改进的Gompertz和Logistic模型用于成功预测氢气的累积产量。