在高静水压力下降低 TCA 循环速率会阻碍天然深海微生物群落中的碳氢化合物降解并强制油降解剂。,The ISME Journal

当前位置： X-MOL 学术 › ISME J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

在高静水压力下降低 TCA 循环速率会阻碍天然深海微生物群落中的碳氢化合物降解并强制油降解剂。
The ISME Journal ( IF 10.8 ) Pub Date : 2018-12-12 , DOI: 10.1038/s41396-018-0324-5
Alberto Scoma _{1,

2,

3} , Robert Heyer ₄ , Ridwan Rifai ₁ , Christian Dandyk ₄ , Ian Marshall ₂ , Frederiek-Maarten Kerckhof ₁ , Angeliki Marietou ₂ , Henricus T S Boshker _{5,

6} , Filip J R Meysman _{5,

6,

7} , Kirsten G Malmos ₈ , Thomas Vosegaard ₈ , Pieter Vermeir ₉ , Ibrahim M Banat ₁₀ , Dirk Benndorf _{4,

11} , Nico Boon ₁

Affiliation

Center for Microbial Ecology and Technology (CMET), Gent University, Coupure Links 653, Gent, B 9000, Belgium.
Department of Bioscience, Microbiology Section, Aarhus University, Ny Munkegade 116, Aarhus C, 8000, Denmark.
Biological and Chemical Engineering, Aarhus University, Hangøvej 2, Aarhus N, 8200, Denmark.
Otto von Guericke University of Magdeburg, Bioprocess Engineering, Universitätsplatz 2 G25, Magdeburg, 39106, Germany.
Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, Delft, 2629HZ, The Netherlands.
Department of Biology, University of Antwerp, Universiteitsplein 1, Wilrijk (Antwerp), BE- 2610, Belgium.
Department of Analytical, Environmental and Geochemistry (AMGC), Vrije Univeriteit Brussel (VUB), Pleinlaan 2, Brussel, 1050, Belgium.
iNANO, Department of Chemistry, Aarhus University, Gustav Wieds vej 14, Aarhus C, 8000, Denmark.
Laboratory for Chemical Analyses (LCA), Department of Green Chemistry and Technology, Gent University, Valentin Vaerwyckweg 1, Ghent, 9000, Belgium.
School of Biomedical Sciences, University of Ulster, Coleraine, N. Ireland, UK.
Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstraße 1, Magdeburg, 39106, Germany.

石油烃在自然和人为因素的作用下到达深海。它们进入深海微生物食物网并影响碳和其他元素的生物地球化学循环的过程尚不清楚。静水压力 (HP) 是深海的一个独特参数，尽管很少研究。HP是否单独影响石油降解社区的集会和活动仍有待解决。在这里，我们已经证明深海微生物群落中的碳氢化合物降解在天然高压（10 MPa，海面以下约 1000 m）下低于环境压力。在长期富集过程中，增加的 HP 选择性地抑制专性烃降解物并下调 β-氧化相关蛋白的表达（即，主要的碳氢化合物降解途径）导致低细胞生长和 CO2 产生。与 HP 适应的合成群落的短期实验证实了这一数据，揭示了柠檬酸盐和二羟基丙酮的 HP 依赖性积累。柠檬酸盐的积累表明 TCA 循环中脂肪酸的有氧氧化率降低了。二羟基丙酮通过甘油代谢和糖酵解与柠檬酸盐相连，两者都随着 HP 增加而上调。因此，专性碳氢化合物降解剂的高降解率可能在 HP 增加时不利，这解释了它们的选择性抑制。通过实验室规模的培养，本研究首次强调了在高 HP 条件下碳氢化合物降解中受损的细胞代谢与微生物群落组装之间的联系。总体，

"点击查看英文标题和摘要"

Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities.

Petroleum hydrocarbons reach the deep-sea following natural and anthropogenic factors. The process by which they enter deep-sea microbial food webs and impact the biogeochemical cycling of carbon and other elements is unclear. Hydrostatic pressure (HP) is a distinctive parameter of the deep sea, although rarely investigated. Whether HP alone affects the assembly and activity of oil-degrading communities remains to be resolved. Here we have demonstrated that hydrocarbon degradation in deep-sea microbial communities is lower at native HP (10 MPa, about 1000 m below sea surface level) than at ambient pressure. In long-term enrichments, increased HP selectively inhibited obligate hydrocarbon-degraders and downregulated the expression of beta-oxidation-related proteins (i.e., the main hydrocarbon-degradation pathway) resulting in low cell growth and CO2 production. Short-term experiments with HP-adapted synthetic communities confirmed this data, revealing a HP-dependent accumulation of citrate and dihydroxyacetone. Citrate accumulation suggests rates of aerobic oxidation of fatty acids in the TCA cycle were reduced. Dihydroxyacetone is connected to citrate through glycerol metabolism and glycolysis, both upregulated with increased HP. High degradation rates by obligate hydrocarbon-degraders may thus be unfavourable at increased HP, explaining their selective suppression. Through lab-scale cultivation, the present study is the first to highlight a link between impaired cell metabolism and microbial community assembly in hydrocarbon degradation at high HP. Overall, this data indicate that hydrocarbons fate differs substantially in surface waters as compared to deep-sea environments, with in situ low temperature and limited nutrients availability expected to further prolong hydrocarbons persistence at deep sea.

更新日期：2019-01-25

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文