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Fatty Acid- and Amino Acid-Specific Isotope Analysis for Accurate Authentication and Traceability in Organic Milk
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-13 00:00:00 , DOI: 10.1021/acs.jafc.8b05063 Ill-Min Chung 1 , Jae-Kwang Kim 2 , Christopher T. Yarnes 3 , Yeon-Ju An 1 , Chang Kwon 1 , So-Yeon Kim 1 , Yu-Jin Yang 1 , Hee-Youn Chi 1 , Seung-Hyun Kim 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-12-13 00:00:00 , DOI: 10.1021/acs.jafc.8b05063 Ill-Min Chung 1 , Jae-Kwang Kim 2 , Christopher T. Yarnes 3 , Yeon-Ju An 1 , Chang Kwon 1 , So-Yeon Kim 1 , Yu-Jin Yang 1 , Hee-Youn Chi 1 , Seung-Hyun Kim 1
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The present study describes compound-specific δ13C and δ15N analyses of fatty acids and amino acids for improving the accurate authentication of organic milk (OM) against conventional milk (CM) collected in Korea. Most δ13Cfatty-acid and δ13Camino-acid values were lower in OM than in CM (P < 0.05); however, most δ15Namino-acid values displayed weak discriminative power for OM authentication. Higher isotopic fractionation was observed in δ13Cfatty-acid than in δ13Camino-acid and δ15Namino-acid, with fractionation trends differing with individual amino acids. In particular, δ13Clinoleic-acid of −33.5‰ and δ13Cmyristic-acid of −28‰ were determined to be promising year-round threshold values for Korean OM authentication. The δ13Cbulk was highly correlated with δ13CAla (r = 0.92) and δ13Coleic-acid,trans (r = 0.77), and strong positive correlations were observed between δ13CVal and δ13CIle (r = 0.98) and between δ15NThr and δ15NSer (r = 0.90). Chemometric modeling for OM authentication produced a high quality model (R2X = 0.547, R2Y = 0.865, and Q2 = 0.689) with reliable chemical markers, notably δ13Cmyristic-acid, δ13Clinoleic-acid, and δ13Cstearic-acid. Furthermore, the models developed for seasonal separation in OM (Q2 = 0.954) and CM (Q2 = 0.791) were of good quality. Our findings, based on compound-specific isotope data, improve the reliability of OM authentication in cases where bulk stable isotope ratio analysis alone is insufficient. They also provide valuable insight into the control of fraudulent OM labeling in Korea, with potential application in other countries.
中文翻译：
脂肪酸和氨基酸特定的同位素分析，可对有机牛奶进行准确的鉴定和追溯
本研究中描述了化合物的特定δ 13 C和δ 15在韩国收集脂肪酸和氨基酸用于改善有机牛奶（OM）针对常规牛奶（CM）的高精度的认证Ñ分析。最δ 13 C ^脂肪酸和δ 13 Ç氨基酸值在OM比CM降低（P <0.05）; 然而，大多数δ 15 Ñ氨基酸值显示弱辨别力为OM验证。更高于δ观察到的同位素分馏13 Ç脂肪酸比δ 13 Ç氨基酸和δ 15N氨基酸，分馏趋势随单个氨基酸的不同而不同。特别地，δ 13 Ç亚油酸的-33.5‰和δ 13 C ^肉豆蔻酸-28测定韩国OM认证是有前途全年阈值‰。的δ 13 C ^散装物高度与δ相关13 Ç丙氨酸（[R = 0.92）和δ 13 Ç油酸酸，反式（- [R δ之间观察= 0.77），和强正相关13 Ç缬氨酸和δ 13 C ^ILE（[R = 0.98）和δ之间15 Ñ苏氨酸和δ 15 Ñ丝氨酸（[R = 0.90）。对于OM认证的化学计量学建模产生的高品质模式（- [R 2 X = 0.547，[R 2 ÿ = 0.865，和Q 2以可靠的化学标记物，特别是δ= 0.689）13 Ç肉豆蔻酸，δ 13 Ç亚油酸，和δ 13 ç硬脂酸酸。此外，针对OM中的季节性分离而开发的模型（Q 2= 0.954）和CM（Q 2 = 0.791）质量良好。我们的发现基于化合物特定的同位素数据，在仅进行整体稳定同位素比分析不足的情况下，可提高OM验证的可靠性。他们还提供了对韩国欺诈性OM标签控制的宝贵见解，并有可能在其他国家/地区应用。
更新日期：2018-12-13
中文翻译：
脂肪酸和氨基酸特定的同位素分析，可对有机牛奶进行准确的鉴定和追溯
本研究中描述了化合物的特定δ 13 C和δ 15在韩国收集脂肪酸和氨基酸用于改善有机牛奶（OM）针对常规牛奶（CM）的高精度的认证Ñ分析。最δ 13 C ^脂肪酸和δ 13 Ç氨基酸值在OM比CM降低（P <0.05）; 然而，大多数δ 15 Ñ氨基酸值显示弱辨别力为OM验证。更高于δ观察到的同位素分馏13 Ç脂肪酸比δ 13 Ç氨基酸和δ 15N氨基酸，分馏趋势随单个氨基酸的不同而不同。特别地，δ 13 Ç亚油酸的-33.5‰和δ 13 C ^肉豆蔻酸-28测定韩国OM认证是有前途全年阈值‰。的δ 13 C ^散装物高度与δ相关13 Ç丙氨酸（[R = 0.92）和δ 13 Ç油酸酸，反式（- [R δ之间观察= 0.77），和强正相关13 Ç缬氨酸和δ 13 C ^ILE（[R = 0.98）和δ之间15 Ñ苏氨酸和δ 15 Ñ丝氨酸（[R = 0.90）。对于OM认证的化学计量学建模产生的高品质模式（- [R 2 X = 0.547，[R 2 ÿ = 0.865，和Q 2以可靠的化学标记物，特别是δ= 0.689）13 Ç肉豆蔻酸，δ 13 Ç亚油酸，和δ 13 ç硬脂酸酸。此外，针对OM中的季节性分离而开发的模型（Q 2= 0.954）和CM（Q 2 = 0.791）质量良好。我们的发现基于化合物特定的同位素数据，在仅进行整体稳定同位素比分析不足的情况下，可提高OM验证的可靠性。他们还提供了对韩国欺诈性OM标签控制的宝贵见解，并有可能在其他国家/地区应用。
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