Poly(vinyl alcohol)-induced thixotropy of an l-carnosine-based cytocompatible, tripeptidic hydrogel†,Soft Matter

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Poly(vinyl alcohol)-induced thixotropy of an l-carnosine-based cytocompatible, tripeptidic hydrogel†
Soft Matter ( IF 2.9 ) Pub Date : 2018-12-07 00:00:00 , DOI: 10.1039/c8sm01766b
Rita Das Mahapatra _{1,

2,

3,

4} , Joykrishna Dey _{1,

2,

3,

4} , Richard G. Weiss _{5,

6,

7,

8}

Affiliation

The generally poor mechanical stability of hydrogels limits their use as functional materials for many biomedical applications. In this work, a poly(vinyl alcohol) (PVA) embedded hybrid hydrogel of a β-amino acid-containing Fmoc-protected tripeptide was produced at physiological pH (7.4) and room temperature. The hydrogel system was characterized by a number of techniques, including UV-vis, fluorescence, circular dichroism, FT-IR spectroscopy, electron microscopy, and rheology. While the tripeptide-based pure hydrogel was found to be unstable after ca. half an hour, addition of PVA, a water soluble polymer, increased the temporal and mechanical stability of the hydrogel. A rheological step-strain experiment demonstrates that the peptide-polymer hydrogel is thixotropic. Results from a fluorescence probe study and transmission electron microscopy reveal that addition of PVA increases both the fibre diameter and entanglement. Circular dichroism spectra of the hydrogels confirm the formation of aggregates with supramolecular chirality. The thixotropic nature of the hydrogel has been exploited to entrap and release doxorubicin, an anticancer drug, under physiological conditions. Furthermore, an MTT assay of the Fmoc-tripeptide using AH927 cells confirmed its cytocompatibility, which broadens the utility of the hybrid gel for biomedical applications.

中文翻译：

聚（乙烯醇）诱发的触变性的升基于-carnosine-细胞相容性，水凝胶tripeptidic †

水凝胶通常较差的机械稳定性限制了它们作为许多生物医学应用的功能材料的用途。在这项工作中，在生理pH（7.4）和室温下，制备了含β-氨基酸的Fmoc保护的三肽的聚乙烯醇（PVA）嵌入杂化水凝胶。水凝胶系统的特征在于多种技术，包括紫外可见光谱，荧光，圆二色性，傅立叶变换红外光谱，电子显微镜和流变学。虽然发现三肽基纯水凝胶在约200℃后不稳定。半小时后，添加水溶性聚合物PVA可以提高水凝胶的时间和机械稳定性。流变分步应变实验表明，肽-聚合物水凝胶是触变的。荧光探针研究和透射电子显微镜的结果表明，添加PVA会增加纤维直径和缠结度。水凝胶的圆二色性光谱证实了具有超分子手性的聚集体的形成。水凝胶的触变性已被开发出来，可以在生理条件下截留和释放阿霉素（一种抗癌药）。此外，使用AH927细胞对Fmoc-三肽进行的MTT分析证实了其细胞相容性，这拓宽了杂化凝胶在生物医学应用中的用途。

更新日期：2018-12-07

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