A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction,The Analyst

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A novel fluorescence method for activity assay and drug screening of T4 PNK by coupling rGO with ligase reaction
The Analyst Pub Date : 2018-12-05 , DOI: 10.1039/c8an02147c
Hongyan Zhou _{1,

2,

3,

4} , Chunyi Tong _{1,

2,

3,

4} , Wei Zou _{5,

6,

7,

8,

9} , Yupei Yang _{3,

10,

11,

12,

13} , Yongbei Liu _{3,

10,

11,

12,

13} , Bin Li _{3,

10,

11,

12,

13} , Yan Qin _{3,

10,

11,

12,

13} , Wenya Dang _{1,

2,

3,

4} , Bin Liu _{1,

2,

3,

4,

10} , Wei Wang _{3,

10,

11,

12,

13}

Affiliation

T4 polynucleotide kinase (PNK) is the primary member of the 5′-kinase family that can transfer the γ-phosphate residue of ATP to the 5′-hydroxyl group of oligonucleotides.

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