Regeneration of complex multi-tissue structures, such as limbs, requires the coordinated effort of multiple cell types. In axolotl limb regeneration, the wound epidermis and blastema have been extensively studied via histology, grafting, and bulk-tissue RNA-sequencing. However, defining the contributions of these tissues is hindered due to limited information regarding the molecular identity of the cell types in regenerating limbs. Here we report unbiased single-cell RNA-sequencing on over 25,000 cells from axolotl limbs and identify a plethora of cellular diversity within epidermal, mesenchymal, and hematopoietic lineages in homeostatic and regenerating limbs. We identify regeneration-induced genes, develop putative trajectories for blastema cell differentiation, and propose the molecular identity of fibroblast-like blastema progenitor cells. This work will enable application of molecular techniques to assess the contribution of these populations to limb regeneration. Overall, these data allow for establishment of a putative framework for adult axolotl limb regeneration.

中文翻译：

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在单细胞分辨率下再生成年a肢体中胚泡生态位的转录组学景观。

复杂的多组织结构（如四肢）的再生需要多种细胞类型的协同努力。在a的肢体再生中，已通过组织学，移植和大组织RNA测序对伤口表皮和胚细胞进行了广泛研究。但是，由于有关再生肢体中细胞类型的分子身份的信息有限，因此难以定义这些组织的作用。在这里，我们报道了来自腋臭肢体的25,000多个细胞的无偏差单细胞RNA测序，并在稳态和再生肢体的表皮，间充质和造血谱系中发现了过多的细胞多样性。我们确定再生诱导的基因，发展为胚细胞分化的假定轨迹，并提出成纤维细胞样胚细胞祖细胞的分子身份。这项工作将使分子技术的应用能够评估这些人群对肢体再生的贡献。总的来说，这些数据可以为成人a的肢体再生建立一个推定的框架。