Facile Preparation of a Bacteria Imprinted Artificial Receptor for Highly Selective Bacterial Recognition and Label-Free Impedimetric Detection.,Analytical Chemistry

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Facile Preparation of a Bacteria Imprinted Artificial Receptor for Highly Selective Bacterial Recognition and Label-Free Impedimetric Detection.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-12-03 00:00:00 , DOI: 10.1021/acs.analchem.8b04314
Jikui Wu ₁ , Ruinan Wang ₁ , Yunfei Lu ₁ , Min Jia ₁ , Juan Yan ₁ , Xiaojun Bian _{1,

2,

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Affiliation

The effective identification and quantification of pathogenic bacteria is essential for addressing serious public health issues. Here, we demonstrate a simple and universal impedimetric sensor for highly selective and sensitive detection of pathogenic bacteria based on the recognition by a bacteria-imprinted polypyrrole (BIP) film. The BIP film was facilely prepared via one-step electro-polymerization followed by in situ removal of the bacterial template. The film structure is novel with noncavity-like imprinted sites situated at the surface of the polypyrrole (PPy) matrix, which are more accessible for the target bacteria and should enhance the mass transfer and the binding kinetics. A limit of quantitation low to 10³ CFU/mL was achieved within 1 h for the detection of E. coli O157:H7, which is comparable to the antibody-based assays. Moreover, the sensor displayed remarkable selectivity, especially regarding the specific identification of bacterial serotypes. When employed to analyze E. coli O157:H7 in real drinking water, apple juice, and milk samples, the sensor showed recoveries from 96.0% to 107.9% with relative standard derivations (RSDs) less than 4%. The BIP-based sensing strategy provides a universal approach for specific, selective, and rapid detection of pathogenic bacteria. As compared to conventional biosensors based on biomolecular recognition, this sensor shows clear advantages including easy-of-preparation, robustness, and low cost, which may hold great potential in fields of food/public safety monitoring.

中文翻译：

轻松制备用于高选择性细菌识别和无标签阻抗测定的细菌印迹人工受体。

有效鉴定和定量病原细菌对于解决严重的公共卫生问题至关重要。在这里，我们展示了一种基于细菌印迹聚吡咯（BIP）膜的识别功能的，用于病原菌的高度选择性和灵敏检测的简单通用的阻抗传感器。通过一步电聚合，然后原位去除细菌模板，可以轻松地制备BIP膜。该膜结构是新颖的，在聚吡咯（PPy）基质的表面上有非腔状的印迹部位，靶细菌更容易接近该部位，并应增强传质和结合动力学。在1 h内检测到大肠杆菌的定量限低至10 ³ CFU / mLO157：H7，与基于抗体的测定相当。此外，该传感器显示出显着的选择性，特别是在细菌血清型的特异性鉴定方面。当用于分析实际饮用水，苹果汁和牛奶样品中的大肠杆菌O157：H7时，该传感器的回收率从96.0％增至107.9％，相对标准偏差（RSD）小于4％。基于BIP的传感策略为特异性，选择性和快速地检测病原菌提供了一种通用方法。与基于生物分子识别的常规生物传感器相比，该传感器具有明显的优势，包括易于制备，坚固耐用和成本低廉，在食品/公共安全监控领域可能具有巨大潜力。

更新日期：2018-12-03

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