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Brain-Specific Ultrastructure of Capillary Endothelial Glycocalyx and Its Possible Contribution for Blood Brain Barrier.
Scientific Reports ( IF 3.8 ) Pub Date : 2018-11-30 , DOI: 10.1038/s41598-018-35976-2 Yoshiaki Ando 1 , Hideshi Okada 1 , Genzou Takemura 2 , Kodai Suzuki 1 , Chihiro Takada 1 , Hiroyuki Tomita 3 , Ryogen Zaikokuji 4 , Yasuaki Hotta 5 , Nagisa Miyazaki 2 , Hirohisa Yano 1 , Isamu Muraki 1 , Ayumi Kuroda 1 , Hirotsugu Fukuda 1 , Yuki Kawasaki 1 , Haruka Okamoto 1 , Tomonori Kawaguchi 1 , Takatomo Watanabe 6 , Tomoaki Doi 1 , Takahiro Yoshida 1 , Hiroaki Ushikoshi 1 , Shozo Yoshida 1 , Shinji Ogura 1
Scientific Reports ( IF 3.8 ) Pub Date : 2018-11-30 , DOI: 10.1038/s41598-018-35976-2 Yoshiaki Ando 1 , Hideshi Okada 1 , Genzou Takemura 2 , Kodai Suzuki 1 , Chihiro Takada 1 , Hiroyuki Tomita 3 , Ryogen Zaikokuji 4 , Yasuaki Hotta 5 , Nagisa Miyazaki 2 , Hirohisa Yano 1 , Isamu Muraki 1 , Ayumi Kuroda 1 , Hirotsugu Fukuda 1 , Yuki Kawasaki 1 , Haruka Okamoto 1 , Tomonori Kawaguchi 1 , Takatomo Watanabe 6 , Tomoaki Doi 1 , Takahiro Yoshida 1 , Hiroaki Ushikoshi 1 , Shozo Yoshida 1 , Shinji Ogura 1
Affiliation
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Endothelial glycocalyx coats healthy vascular endothelium and plays an important role in vascular homeostasis. Although cerebral capillaries are categorized as continuous, as are those in the heart and lung, they likely have specific features related to their function in the blood brain barrier. To test that idea, brains, hearts and lungs from C57BL6 mice were processed with lanthanum-containing alkaline fixative, which preserves the structure of glycocalyx, and examined using scanning and transmission electron microscopy. We found that endothelial glycocalyx is present over the entire luminal surface of cerebral capillaries. The percent area physically covered by glycocalyx within the lumen of cerebral capillaries was 40.1 ± 4.5%, which is significantly more than in cardiac and pulmonary capillaries (15.1 ± 3.7% and 3.7 ± 0.3%, respectively). Upon lipopolysaccharide-induced vascular injury, the endothelial glycocalyx was reduced within cerebral capillaries, but substantial amounts remained. By contrast, cardiac and pulmonary capillaries became nearly devoid of glycocalyx. These findings suggest the denser structure of glycocalyx in the brain is associated with endothelial protection and may be an important component of the blood brain barrier.
中文翻译：
毛细血管内皮糖萼的脑特异性超微结构及其对血脑屏障的可能贡献。
内皮糖萼覆盖着健康的血管内皮，并在血管稳态中发挥重要作用。尽管脑毛细血管和心脏和肺毛细血管都被分类为连续毛细血管，但它们可能具有与其在血脑屏障中的功能有关的特定特征。为了验证该想法，将C57BL6小鼠的大脑，心脏和肺部用含镧的碱性固定剂处理，该固定剂保留了糖萼的结构，并使用扫描和透射电子显微镜进行了检查。我们发现内皮糖萼存在于脑毛细血管的整个腔表面。糖萼物理覆盖的脑毛细血管腔内的面积百分比为40.1±4.5％，显着高于心脏和肺毛细血管（分别为15.1±3.7％和3.7±0.3％）。脂多糖诱导的血管损伤后，脑毛细血管内的内皮糖萼减少，但仍有大量残留。相反，心脏和肺毛细血管几乎没有糖萼。这些发现表明，大脑中糖萼的致密结构与内皮保护有关，可能是血脑屏障的重要组成部分。
更新日期：2018-11-30
中文翻译：
毛细血管内皮糖萼的脑特异性超微结构及其对血脑屏障的可能贡献。
内皮糖萼覆盖着健康的血管内皮，并在血管稳态中发挥重要作用。尽管脑毛细血管和心脏和肺毛细血管都被分类为连续毛细血管，但它们可能具有与其在血脑屏障中的功能有关的特定特征。为了验证该想法，将C57BL6小鼠的大脑，心脏和肺部用含镧的碱性固定剂处理，该固定剂保留了糖萼的结构，并使用扫描和透射电子显微镜进行了检查。我们发现内皮糖萼存在于脑毛细血管的整个腔表面。糖萼物理覆盖的脑毛细血管腔内的面积百分比为40.1±4.5％，显着高于心脏和肺毛细血管（分别为15.1±3.7％和3.7±0.3％）。脂多糖诱导的血管损伤后，脑毛细血管内的内皮糖萼减少，但仍有大量残留。相反，心脏和肺毛细血管几乎没有糖萼。这些发现表明，大脑中糖萼的致密结构与内皮保护有关，可能是血脑屏障的重要组成部分。
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