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Enhanced Osteogenesis of Bone Marrow‐Derived Mesenchymal Stem Cells by a Functionalized Silk Fibroin Hydrogel for Bone Defect Repair
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-11-28 , DOI: 10.1002/adhm.201801043 Yufei Yan 1 , Baochang Cheng 2 , Kaizhe Chen 1 , Wenguo Cui 1 , Jin Qi 1 , Xinming Li 2 , Lianfu Deng 1
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2018-11-28 , DOI: 10.1002/adhm.201801043 Yufei Yan 1 , Baochang Cheng 2 , Kaizhe Chen 1 , Wenguo Cui 1 , Jin Qi 1 , Xinming Li 2 , Lianfu Deng 1
Affiliation
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Silk fibroin (SF) from Bombyx mori is a promising natural material for the synthesis of biocompatible and biodegradable hydrogels for use in biomedical applications from tissue engineering to drug delivery. However, weak gelation performance and the lack of biochemical cues to trigger cell proliferation and differentiation currently significantly limit its application in these areas. Herein, a biofunctional hydrogel containing SF (2.0%) and a small peptide gelator (e.g., NapFFRGD = 1.0 wt%) is generated via cooperative molecular self‐assembly. The introduction of NapFFRGD to SF is shown to significantly improve its gelation properties by lowering both its threshold gelation concentration to 2.0% and gelation time to 20 min under physiological conditions (pH = 7.4, 37 °C), as well as functionalizing the SF hydrogel with cell‐adhesive motifs (e.g., RGD). Besides mediating cell adhesion, the RGD ligands incorporated within the SF‐RGD gel promote the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells encapsulated within the gel matrix, leading to bone regeneration in a mouse calvarial defect model, compared with a blank SF gel (2.0%, pH = 7.4). This work suggests that SF could be easily tailored with bioactive peptide gelators to afford bioactive hydrogels with favorable microenvironments for tissue regeneration applications.
中文翻译：
功能化的丝素蛋白水凝胶对骨缺损修复的作用增强了骨髓间充质干细胞的成骨作用。
蚕丝素（SF）从家蚕是用于合成生物相容性和可生物降解水凝胶的有前途的天然材料，可用于从组织工程到药物输送的生物医学应用。然而，弱的胶凝性能和缺乏触发细胞增殖和分化的生化线索目前严重限制了其在这些领域的应用。在此，通过协同分子自组装产生了包含SF（2.0％）和小肽凝胶剂（例如，NapFFRGD = 1.0 wt％）的生物功能水凝胶。将NapFFRGD引入SF可通过在生理条件下（pH = 7.4，37°C）将其阈值胶凝浓度降低至2.0％和将胶凝时间降低至20分钟，以及功能化SF水凝胶来显着改善其胶凝性能。具有细胞粘附基序（例如RGD）。除了介导细胞黏附外，SF-RGD凝胶中掺入的RGD配体还促进了封装在凝胶基质中的骨髓间充质干细胞的成骨分化，与空白SF凝胶相比，可导致小鼠颅盖缺损模型的骨再生。 （2.0％，pH ＝ 7.4）。这项工作表明，可以使用生物活性肽胶凝剂轻松定制SF，从而为组织再生应用提供具有良好微环境的生物活性水凝胶。
更新日期：2018-11-28
中文翻译：
功能化的丝素蛋白水凝胶对骨缺损修复的作用增强了骨髓间充质干细胞的成骨作用。
蚕丝素（SF）从家蚕是用于合成生物相容性和可生物降解水凝胶的有前途的天然材料，可用于从组织工程到药物输送的生物医学应用。然而，弱的胶凝性能和缺乏触发细胞增殖和分化的生化线索目前严重限制了其在这些领域的应用。在此，通过协同分子自组装产生了包含SF（2.0％）和小肽凝胶剂（例如，NapFFRGD = 1.0 wt％）的生物功能水凝胶。将NapFFRGD引入SF可通过在生理条件下（pH = 7.4，37°C）将其阈值胶凝浓度降低至2.0％和将胶凝时间降低至20分钟，以及功能化SF水凝胶来显着改善其胶凝性能。具有细胞粘附基序（例如RGD）。除了介导细胞黏附外，SF-RGD凝胶中掺入的RGD配体还促进了封装在凝胶基质中的骨髓间充质干细胞的成骨分化，与空白SF凝胶相比，可导致小鼠颅盖缺损模型的骨再生。 （2.0％，pH ＝ 7.4）。这项工作表明，可以使用生物活性肽胶凝剂轻松定制SF，从而为组织再生应用提供具有良好微环境的生物活性水凝胶。
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