Twenty-five white-tailed deer (Odocoileus virginianus yucatanensis) (WTD), 4 mazama deer (Mazama temama) (MD), and the ticks they host in Yucatan, Mexico were sampled to run a molecular survey for tick-borne rickettsial agents. The prevalence of rickettsial agents was 20% in WTD (5/25) and 50% in MD (2/4). When sequencing the nested PCR products, E. chaffeensis, A. phagocytophilum and A. odocoilei, were identified as single infection or coinfecting cervids. None of the cervid samples were positive for E. ewingii, E. canis, nor Rickettsia spp. Overall, 355 individual ticks were collected. Species identified based on adult stages infesting cervids included Amblyomma mixtum, A. parvum, A. cf. oblongoguttatum, Ixodes affinis, Rhipicephalus microplus, R. sanguineus sensu lato, and Haemaphysalis juxtakochi. Rhipicephalus microplus was the tick species most commonly found infesting cervids with a frequency of 28.4%, and intensity of 25.2 ticks per animal. A pool of Amblyomma cf. oblongoguttatum adults and one of Amblyomma spp. nymphs were positive for E. canis and E. chaffeensis, respectively. None of the studied tick pools were positive for E. ewingii, A. phagocytophilum, nor R. rickettsii. To the best of our knowledge, this study is the first to report the prevalence of rickettsial agents in WTD and MD in Mexico. Our molecular study is the first to report the detection of E. chaffeensis, A. phagocytophilum, and A. odocoilei in MD in Mexico. The molecular detection of E. chaffeensis, A. phagocytophilum, and A. odocoilei in deer, and E. chaffeensis in Amblyomma spp. nymphs reported here raises the concern for the risk of human exposure to tick-borne rickettsial pathogens. Our findings highlight the need to apply the “One Health” approach to study ticks and tick-borne diseases. This science-based information could be used by state public-health programs to assess the risk for exposure to tick-borne Anaplasmataceae in Yucatan, Mexico.

对25只白尾鹿（Odocoileus virginianus yucatanensis）（WTD），4座马（Mazama temama）（MD）以及它们在墨西哥尤卡坦的host进行采样，以对tick传播的病进行分子调查。立克次体病的患病率在WTD（5/25）中为20％，在MD（2/4）中为50％。在对巢式PCR产物进行测序时，发现恰菲埃里希氏菌，吞噬嗜热芽孢杆菌和odocoilei隐窝菌是单一感染或共感染的宫颈。cervid样品中的任何一种均未对Ewinging E.， Canis E. canis和Rickettsia spp呈阳性。总体上，收集了355个单独的滴答声。根据成年阶段侵染的子宫颈动物鉴定出的物种花蜱mixtum，A.孢子虫，A.比照。oblongoguttatum，硬蜱AFFINIS，扇头蜱microplus，血红扇头意义上拉托，和血蜱juxtakochi。Rhipicephalus microplus是最常见的侵扰性子宫颈the科物种，其频率为28.4％，每只动物的s虫强度为25.2。Amblyomma池cf. oblongoguttatum成人和Amblyomma spp之一。若虫对犬埃里希体和恰菲埃里希体分别呈阳性。所研究的滴答池均无阳性E. ewingii，A。phagocytophilum或R. rickettsii。据我们所知，该研究是第一个报告了在WTD和MD中墨西哥的ett病病患的流行情况。我们的分子研究是首次报道的检测恰菲埃里希氏，A.吞噬细胞，和A. odocoilei在墨西哥MD。的分子检测恰菲埃里希氏体，A.吞噬细胞，和A. odocoilei在鹿和恰菲埃里希氏体在花蜱spp。这里报道的若虫引起了人们对tick传播的立克次氏病病原体的暴露风险的担忧。我们的研究结果突出表明，有必要应用“单一健康”方法来研究tick和tick传播的疾病。州公共卫生计划可以使用这种基于科学的信息来评估暴露于墨西哥尤卡坦州tick传播的无形纲科的风险。