脑卒中模型脑内注射后工程自组装丝素蛋白水凝胶的体内评价,ACS Biomaterials Science & Engineering

当前位置： X-MOL 学术 › ACS Biomater. Sci. Eng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

脑卒中模型脑内注射后工程自组装丝素蛋白水凝胶的体内评价
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2018-11-27 00:00:00 , DOI: 10.1021/acsbiomaterials.8b01024
Natalia Gorenkova ₁ , Ibrahim Osama ₁ , F. Philipp Seib _{1,

2} , Hilary V.O. Carswell ₁

Affiliation

以缺血性中风形成的脑腔为目标吸引许多再生治疗策略，但需要强大的输送技术。我们假设自组装的丝素蛋白水凝胶可以作为中风腔再生的可靠支持基质。因此，我们在大鼠中风模型中进行了脑内注射后自我组装的丝素蛋白水凝胶的体内评估研究。成年雄性Sprague–Dawley大鼠（n= 24）在随机分配前两周接受短暂脑中动脉闭塞（MCAo），既不进行立体定位注射，也不采用自组装的丝素蛋白水凝胶（4％w / v）或PBS立体定位注射到病变腔内。盲人研究者在长达7周的时间内检查了其对发病率和死亡率，空间整合性，与神经胶质疤痕的相互作用，对炎症反应的干扰以及细胞增殖在病变腔中的影响。自组装水凝胶以优异的空间适应性填充中风腔，既没有明显的小胶质细胞/巨噬细胞反应，也没有不良的发病率或死亡率。注射自组装丝水凝胶可显着改变增殖细胞数与病变体积之间的关系。

"点击查看英文标题和摘要"

In Vivo Evaluation of Engineered Self-Assembling Silk Fibroin Hydrogels after Intracerebral Injection in a Rat Stroke Model

Targeting the brain cavity formed by an ischemic stroke is appealing for many regenerative treatment strategies but requires a robust delivery technology. We hypothesized that self-assembling silk fibroin hydrogels could serve as a reliable support matrix for regeneration in the stroke cavity. We therefore performed in vivo evaluation studies of self-assembling silk fibroin hydrogels after intracerebral injection in a rat stroke model. Adult male Sprague–Dawley rats (n = 24) underwent transient middle cerebral artery occlusion (MCAo) 2 weeks before random assignment to either no stereotaxic injection or a stereotaxic injection of either self-assembling silk fibroin hydrogels (4% w/v) or PBS into the lesion cavity. The impact on morbidity and mortality, space conformity, interaction with glial scar, interference with inflammatory response, and cell proliferation in the lesion cavity were examined for up to 7 weeks by a blinded investigator. Self-assembling hydrogels filled the stroke cavity with excellent space conformity and presented neither an overt microglial/macrophage response nor an adverse morbidity or mortality. The relationship between the number of proliferating cells and lesion volume was significantly changed by injection of self-assembling silk hydrogels. This in vivo stroke model confirmed that self-assembling silk fibroin hydrogels provide a favorable microenvironment as a future support matrix in the stroke cavity.

更新日期：2018-11-27

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南