Tumours comprise a heterogeneous collection of cells with distinct genetic and phenotypic properties that can differentially promote progression, metastasis and drug resistance. Emerging single-cell technologies provide a new opportunity to profile individual cells within tumours and investigate what roles they play in these processes. This Review discusses key technological considerations for single-cell studies in cancer, new findings using single-cell technologies and critical open questions for future applications.

中文翻译：

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单细胞分辨率的肿瘤异质性和转移。

肿瘤包括具有不同遗传和表型特性的异质细胞集合，可以差异地促进其进展，转移和耐药性。新兴的单细胞技术为剖析肿瘤中的单个细胞并研究它们在这些过程中所起的作用提供了新的机会。这篇综述讨论了癌症单细胞研究的关键技术考虑，使用单细胞技术的新发现以及未来应用的关键性开放性问题。