Paper microfluidics has attracted much attention since its first introduction around one decade ago due to the merits such as low cost, ease of fabrication and operation, portability, and facile integration with other devices. The dominant application for paper microfluidics still lies in point-of-care testing (POCT), which holds great promise to provide diagnostic tools to meet the ASSURED criteria. With micro/nanostructures inside, paper substrates provide a natural 3D scaffold to mimic native cellular microenvironments and create excellent biointerfaces for cell analysis applications, such as long-term 3D cell culture, cell capture/phenotyping, and cell-related biochemical analysis (small molecules, protein DNA, etc.). This review summarizes cell-related applications based on various engineered paper microdevices and provides some perspectives for paper microfluidics-based cell analysis.

中文翻译：

---

用于细胞分析的纸质微流控技术。

纸微流体技术自10年前问世以来就受到了广泛的关注，其优点包括成本低，易于制造和操作，可移植性以及与其他设备的便捷集成。纸微流控技术的主要应用仍然是即时检验（POCT），它具有提供满足ASSURED标准的诊断工具的巨大希望。纸质基材内部具有微/纳米结构，可提供天然3D支架以模仿天然细胞微环境，并为细胞分析应用（例如长期3D细胞培养，细胞捕获/表型分析以及与细胞相关的生化分析（小分子））创建出色的生物界面，蛋白质DNA等）。