One of the challenges in the petroleum hydrocarbon contaminated groundwater remediation by oxygen releasing compounds (ORCs) is to identify the remediation mechanism and determine the impact of ORCs on the environment and the intrinsic groundwater microorganisms. In this research, the application of encapsulated magnesium peroxide (MgO₂) nanoparticles in the permeable reactive barrier (PRB) for bioremediation of the groundwater contaminated by toluene and naphthalene was studied in the continuous flow sand-packed plexiglass columns within 50 d experiments. For the biodiversity studies, next generation sequencing (NGS) of the 16S rRNA gene was applied. The results showed that naphthalene was metabolized (within 20 days) faster than toluene (after 30 days) by microorganisms of the aqueous phase. By comparing the contaminant removal in the biotic (which resulted in the complete contaminant removal) and abiotic (around 32% removal for naphthalene and 36% for toluene after 50 d) conditions, the significant role of microorganisms on the decontamination process was proved. Furthermore, the attached microbial communities on the porous media were visualized by scanning electron microscopy (SEM). Microbial community structure analysis by NGS technique revealed that the microbial species which were able to degrade toluene and naphthalene such as P. putida and P. mendocina respectively were stimulated by addition of MgO₂ nanoparticles. The presented study resulted in a momentous insight into the application of MgO₂ nanoparticles in the hydrocarbon compounds removal from groundwater.

用氧气释放化合物（ORC）修复石油烃污染的地下水的挑战之一是确定修复机理并确定ORC对环境和固有的地下水微生物的影响。在这项研究中，封装的过氧化镁（MgO ₂在连续流动填充沙子的有机玻璃柱中进行了50天的实验，研究了可渗透反应性屏障（PRB）中的纳米颗粒用于生物修复甲苯和萘污染的地下水。对于生物多样性研究，应用了16S rRNA基因的下一代测序（NGS）。结果表明，萘被水相微生物代谢（20天之内）比甲苯（30天后）更快。通过比较生物中（完全去除污染物）和非生物（50 d后萘去除32％，甲苯去除36％）条件下的污染物去除，证明了微生物在净化过程中的重要作用。此外，通过扫描电子显微镜（SEM）可以观察到多孔介质上附着的微生物群落。利用NGS技术进行的微生物群落结构分析表明，能够降解甲苯和萘等微生物的微生物种类为：。恶臭假单胞菌和门多萨假单胞菌分别通过添加MgO的刺激₂纳米颗粒。提出的研究对MgO ₂纳米颗粒在去除地下水中的烃类化合物中的应用产生了深刻的了解。