Construction of Synthetic Promoters by Assembling the Sigma Factor Binding −35 and −10 Boxes,Biotechnology Journal

当前位置： X-MOL 学术 › Biotechnol. J. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Construction of Synthetic Promoters by Assembling the Sigma Factor Binding −35 and −10 Boxes
Biotechnology Journal ( IF 3.2 ) Pub Date : 2018-12-07 , DOI: 10.1002/biot.201800298
Yang Wang _{1,

2} , Qingtao Liu _{1,

2} , Huanjiao Weng _{1,

2} , Yanan Shi _{1,

2} , Jian Chen _{1,

2} , Guocheng Du _{1,

2} , Zhen Kang _{1,

2}

Affiliation

Promoter is one of the key elements in regulating gene expression. Many natural or synthetic promoters have been modulated by their cis‐ or tans‐regulatory elements to confer instant gene expression change in responding to designated stimuli. In addition, bacterial cells also engage different sigma factors to control the gene expression network at different growth phases or in response to the changing environment and external stresses. In this study, a set of promoters that assimilate the endogenous regulation of different sigma factors σ⁷⁰, σ³⁸, σ³², and σ²⁴ are synthesized. Promoters are designed to contain two or more kinds of interlocking sigma factor binding sites. The most competitive sigma factors will be automatically selected by the cell to take over the synthetic promoters during the cell growth course. Some of the synthetic promoters exhibit very strong strengths under different conditions, including stationary phase, low temperature, acidic pH, and high osmotic pressure. Comparing to the T7 promoter, synthetic promoter P₂₁₂₈₅ achieved higher yields of L‐asparaginase and acid urease in Escherichia coli. The research not only expands the synthetic biology toolbox but also provide another strategy to design and construct synthetic promoters in prokaryotes.

中文翻译：

通过组装Sigma因子结合-35和-10框构建合成启动子。

启动子是调控基因表达的关键因素之一。许多天然或合成启动子已被其顺式或棕褐色调节元件调节，以响应指定的刺激而赋予即时的基因表达变化。此外，细菌细胞还参与不同的sigma因子，以在不同的生长阶段或响应不断变化的环境和外部压力来控制基因表达网络。在这项研究中，该吸收不同sigma因子的内源性调节的一组启动子的σ ⁷⁰，σ ³⁸，σ ³²，σ ²⁴是合成的。启动子被设计为包含两种或多种互锁的sigma因子结合位点。细胞将自动选择最具竞争力的sigma因子，以在细胞生长过程中接管合成启动子。一些合成促进剂在不同条件下表现出非常强的强度，包括固定相，低温，酸性pH和高渗透压。与T7启动子相比，合成启动子P ₂₁₂₈₅在大肠杆菌中获得了更高的L-天冬酰胺酶和酸性脲酶产量。该研究不仅扩展了合成生物学工具箱，而且为在原核生物中设计和构建合成启动子提供了另一种策略。

更新日期：2018-12-08

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南