Imaging of Receptor Dimers in Zebrafish and Living Cells via Aptamer Recognition and Proximity-Induced Hybridization Chain Reaction,Analytical Chemistry

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Imaging of Receptor Dimers in Zebrafish and Living Cells via Aptamer Recognition and Proximity-Induced Hybridization Chain Reaction
Analytical Chemistry ( IF 6.7 ) Pub Date : 2018-11-16 00:00:00 , DOI: 10.1021/acs.analchem.8b04015
Liping Wang _{1,

2} , Wei Li ₃ , Jin Sun ₃ , Su-Yun Zhang ₄ , Sheng Yang ₄ , Jingying Li ₃ , Juan Li _{1,

2} , Huang-Hao Yang ₁

Affiliation

On cell-membrane surfaces, receptor-protein dimers play fundamental roles in many signaling pathways that are crucial for normal biological processes and cancer development. Efficient and sensitive analysis of receptor dimers in the native environment is highly desirable. Herein, we present a strategy for amplified imaging of receptor dimers in zebrafish and living cells that relies on aptamer recognition and proximity-induced hybridization chain reaction. Taking advantage of specific aptamer recognition and enzyme-free signal amplification, this strategy is successfully applied to the visualization of c-Met-receptor dimers in an HGF-independent or -dependent manner. Therefore, the developed imaging strategy paves the way for further investigation of the dimerization or oligomerization states of cell-surface receptors and their corresponding activation processes in zebrafish and living cells.

中文翻译：

通过适体识别和邻近诱导的杂交链反应成像在斑马鱼和活细胞中的受体二聚体的成像。

在细胞膜表面，受体-蛋白二聚体在许多信号通路中起着基本作用，而这些信号通路对于正常的生物学过程和癌症的发展至关重要。非常需要在天然环境中对受体二聚体进行有效而灵敏的分析。在本文中，我们提出了依赖于适体识别和邻近诱导的杂交链反应的斑马鱼和活细胞中受体二聚体放大成像的策略。利用特异性适体识别和无酶信号放大的优势，该策略已成功应用于以HGF独立或依赖方式显示c-Met受体二聚体。所以，

更新日期：2018-11-16

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