Controlling aflatoxin contamination and propagation of Aspergillus flavus by a soy-fermenting Aspergillus oryzae strain.,Scientific Reports

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Controlling aflatoxin contamination and propagation of Aspergillus flavus by a soy-fermenting Aspergillus oryzae strain.
Scientific Reports ( IF 3.8 ) Pub Date : 2018-Nov-15 , DOI: 10.1038/s41598-018-35246-1
Ahmad F Alshannaq _{1,

2} , John G Gibbons ₃ , Mi-Kyung Lee ₄ , Kap-Hoon Han ₅ , Seung-Beom Hong ₆ , Jae-Hyuk Yu _{2,

7,

8}

Affiliation

Aflatoxins (AFs) are a group of carcinogenic and immunosuppressive mycotoxins that threaten global food safety. Globally, over 4.5 billion people are exposed to unmonitored levels of AFs. Aspergillus flavus is the major source of AF contamination in agricultural crops. One approach to reduce levels of AFs in agricultural commodities is to apply a non-aflatoxigenic competitor, e.g., Afla-Guard, to crop fields. In this study, we demonstrate that the food fermenting Aspergillus oryzae M2040 strain, isolated from Korean Meju (a brick of dry-fermented soybeans), can inhibit aflatoxin B1 (AFB1) production and proliferation of toxigenic A. flavus in lab culture conditions and peanuts. In peanuts, 1% inoculation level of A. oryzae M2040 could effectively displace the toxigenic A. flavus and inhibit AFB1 production. Moreover, cell-free culture filtrate of A. oryzae M2040 effectively inhibited AFB1 production and A. flavus growth, suggesting A. oryzae M2040 secretes inhibitory compounds. Whole genome-based comparative analyses indicate that the A. oryzae M2040 and Afla-Guard genomes are 37.9 and 36.4 Mbp, respectively, with each genome containing ~100 lineage specific genes. Our study establishes the idea of using A. oryzae and/or its cell-free culture fermentate as a potent biocontrol agent to control A. flavus propagation and AF contamination.

中文翻译：

通过大豆发酵米曲霉菌株控制黄曲霉毒素污染和黄曲霉繁殖。

黄曲霉毒素（AF）是一组威胁全球食品安全的致癌和免疫抑制霉菌毒素。在全球范围内，超过 45 亿人暴露于不受监控的房颤水平。黄曲霉是农作物中AF污染的主要来源。降低农产品中 AF 水平的一种方法是在农田中施用非黄曲霉毒素竞争剂，例如 Afla-Guard。在这项研究中，我们证明从韩国 Meju（干发酵大豆砖）中分离出来的食品发酵米曲霉 M2040 菌株可以抑制实验室培养条件下和花生中黄曲霉毒素 B1 (AFB1) 的产生和产毒黄曲霉的增殖。。在花生中，接种1%的米曲霉M2040可以有效取代产毒黄曲霉并抑制AFB1的产生。此外，米曲霉M2040的无细胞培养物滤液有效抑制AFB1产生和黄曲霉生长，表明米曲霉M2040分泌抑制性化合物。基于全基因组的比较分析表明，米曲霉 M2040 和 Afla-Guard 基因组分别为 37.9 和 36.4 Mbp，每个基因组包含约 100 个谱系特异性基因。我们的研究确立了使用米曲霉和/或其无细胞发酵培养物作为有效的生物防治剂来控制黄曲霉繁殖和AF污染的想法。

更新日期：2018-11-15

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